Borrelia burgdorferi Spirochetes That Harbor Only a Portion of the lp28-1 Plasmid Elicit Antibody Responses Detectable with the C sub(6) Test for Lyme Disease

• Published in: Clinical and vaccine immunology 2007-01, Vol.14 (1), p.90-93
• Main Authors: Embers, Monica E, Wormser, Gary P, Schwartz, Ira, Martin, Dale S, Philipp, Mario T
• Format: Article
• Language: English
• Subjects: Borrelia burgdorferi
• ISSN: 1556-6811; 1556-679X

Detection of antibody to C sub(6), a peptide that reproduces the sequence of the sixth invariable region within the central domain of the VlsE protein of Borrelia burgdorferi, is used currently for the serologic diagnosis of Lyme disease in humans. B. burgdorferi isolates taken from infected humans can be categorized into specific genetic subtypes (designated RST1, -2, and -3) by restriction fragment length polymorphisms in the 16S to 23S rRNA spacer sequence. Many of these, usually categorized as RST2, retain only segments of the linear plasmid lp28-1, which encodes VlsE. The VlsE genetic region is retained, but altered expression of this molecule could affect diagnosis by the C sub(6) enzyme-linked immunosorbent assay (ELISA). Serum samples from patients infected with each of the three genotypes and from mice infected with three RST2 isolates were tested with the C sub(6) ELISA. Such isolates elicited marked C sub(6) responses in infected mice. The sensitivity of C sub(6) antibody detection in patients infected with RST2 spirochetes was statistically indistinguishable from detection of RST1 and RST3 infections. These findings demonstrate that diagnosis by C sub(6) ELISA remains effective for infection with all B. burgdorferi genotypes, including those with incomplete lp28-1 plasmids.