The advanced glycation end product Nϵ‐carboxymethyllysine and its precursor glyoxal increase serotonin release from Caco‐2 cells

Advanced glycation end products (AGEs), comprising a highly diverse class of Maillard reaction compounds formed in vivo and during heating processes of foods, have been described in the progression of several degenerative conditions such as Alzheimer's disease and diabetes mellitus. Nϵ‐Carboxym...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular biochemistry 2018-03, Vol.119 (3), p.2731-2741
Main Authors: Holik, Ann‐Katrin, Lieder, Barbara, Kretschy, Nicole, Somoza, Mark M., Ley, Jakob P., Hans, Joachim, Somoza, Veronika
Format: Article
Language:English
Subjects:
Advanced glycosylation end products
Alzheimer's disease
caco‐2 cells
Carboxymethyllysine
Diabetes mellitus
DNA microarrays
Enterocytes
Fatty acids
Food
Food processing
Gene expression
Gene regulation
Genomes
Glucose
glucose uptake
Glycosylation
glyoxal
Intestinal motility
Intestine
Maillard reaction
Neurodegenerative diseases
Nϵ‐carboxymethyllysine
Serotonin
serotonin release
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Advanced glycation end products (AGEs), comprising a highly diverse class of Maillard reaction compounds formed in vivo and during heating processes of foods, have been described in the progression of several degenerative conditions such as Alzheimer's disease and diabetes mellitus. Nϵ‐Carboxymethyllysine (CML) represents a well‐characterized AGE, which is frequently encountered in a Western diet and is known to mediate its cellular effects through binding to the receptor for AGEs (RAGE). As very little is known about the impact of exogenous CML and its precursor, glyoxal, on intestinal cells, a genome‐wide screening using a customized microarray was conducted in fully differentiated Caco‐2 cells. After verification of gene regulation by qPCR, functional assays on fatty acid uptake, glucose uptake, and serotonin release were performed. While only treatment with glyoxal showed a slight impact on fatty acid uptake (P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.26439