Utilization of chemical inducers of resistance and Cryptococcus flavescens OH 182.9 to reduce Fusarium head blight under greenhouse conditions

• Published in: Biological control 2007-09, Vol.42 (3), p.308-315
• Main Authors: Zhang, Shouan, Schisler, David A., Boehm, Michael J., Slininger, Patricia J.
• Format: Article
• Language: English
• Subjects: application rate; application timing; biological control agents; butyric acid; Cryptococcus; Cryptococcus (Hyphomycetes); disease control; disease severity; DL-β-amino-n-butyric acid; fungal antagonists; Fusarium; Gibberella zeae; Induced localized resistance; induced resistance; isonicotinic acid; plant pathogenic fungi; salicylic acid; scab diseases; sodium salicylate; Systemic acquired resistance; Triticum aestivum; Wheat scab
• ISSN: 1049-9644; 1090-2112
• DOI: 10.1016/j.biocontrol.2007.05.020

Four chemicals [salicylic acid (SA), sodium salt of salicylic acid (NaSA), isonicotinic acid (INA), and DL-β-amino- n-butyric acid (BABA)] and the yeast antagonist Cryptococcus flavescens (= C. nodaensis nomen nudum) OH 182.9 were evaluated separately or together for the ability to reduce Fusarium head blight (FHB) of wheat in the greenhouse. When sprayed onto wheat heads at 3 days prior to pathogen challenge with Gibberella zeae, NaSA and INA at 10 mM significantly reduced FHB severity compared to the non-treated disease control. Applied at concentrations of 1 and 5 mM at 3 days before pathogen challenge, NaSA or INA in combination with OH 182.9 did not significantly reduce FHB severity compared to either treatment alone, though the lowest disease severity values frequently were associated with the combination treatments. When sprayed onto wheat heads just beginning to emerge from boot at 10 days prior to pathogen inoculation, NaSA, INA, and BABA at 1 mM significantly reduced FHB severity indicating that induced systemic resistance was at least partially responsible for the reduction of FHB disease. Induced FHB resistance was achieved by treating wheat with INA at concentrations as low as 0.1 mM. In only one instance was 100-kernel weight affected by any chemical or combination of chemicals with OH 182.9 treatment. Data from our studies in the greenhouse suggest that chemical inducers can induce resistance in wheat against FHB, and that further efforts are warranted to explore the potential of improved control of FHB disease by incorporating chemical inducers with the FHB biocontrol agent OH 182.9.