Evidence for circadian rhythms in human trophoblast cell line that persist in hypoxia

Circadian clock governs daily rhythmicity of a number of physiological processes such as reproductive functions. The existence of circadian clocks in the placenta is not clearly established. In order to investigate whether human placenta may function as circadian oscillator, we utilized HTR-8/SVneo...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-01, Vol.378 (1), p.108-111
Main Authors: Frigato, Elena, Lunghi, Laura, Ferretti, Maria Enrica, Biondi, Carla, Bertolucci, Cristiano
Format: Article
Language:English
Subjects:
Cell Line
Circadian
Circadian Rhythm - genetics
Clock genes
Cobalt - toxicity
DNA-Binding Proteins - genetics
Female
Gene Expression
Humans
Hypoxia
Hypoxia - chemically induced
Hypoxia - genetics
Nuclear Proteins - genetics
Oxygen - metabolism
Period Circadian Proteins
Placenta - blood supply
Placenta - metabolism
Placentation
Pregnancy
Transcription Factors - genetics
Trophoblast-derived cell line
Trophoblasts - metabolism
Trophoblasts - physiology
Tumor Suppressor Proteins - genetics
Vascular Endothelial Growth Factor A - genetics
Vegf
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Summary:Circadian clock governs daily rhythmicity of a number of physiological processes such as reproductive functions. The existence of circadian clocks in the placenta is not clearly established. In order to investigate whether human placenta may function as circadian oscillator, we utilized HTR-8/SVneo cells derived from human first-trimester trophoblast. In serum-shocked cells we found circadian expressions for the clock genes Per2 and Dec1 as well as for Dbp, a canonical clock-controlled gene. We obtained similar results for Vegf, a circadian output involved in the control of placental vasculogenesis and trophoblast functions. Interestingly, circadian oscillations persisted and even enhanced in cells experimentally rendered hypoxic with CoCl 2. These results could be explained since the hypoxic milieu of the first-trimester placenta is considered the optimal condition for normal placentation. These data collectively support a possible role for the differential rhythmic expression of Vegf, influenced by circadian clock, in the adjustment of placental vascularization and trophoblast functions to the specific requirements of the different gestational ages.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.11.006