Cyanobacteria and their toxins in treated-water storage reservoirs in Abha city, Saudi Arabia

• Published in: Toxicon (Oxford) 2007-07, Vol.50 (1), p.75-84
• Main Authors: Mohamed, Zakaria A., Al Shehri, Abdulrahman M.
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animal poisons toxicology. Antivenoms; Bacteriology; Biological and medical sciences; Calothrix parietina; Chromatography, High Pressure Liquid; Cyanobacteria; Cyanobacteria - chemistry; Cyanobacteria - isolation & purification; Cyanophyta; Endotoxins; Endotoxins - analysis; Enzyme-Linked Immunosorbent Assay; Freshwater; Fundamental and applied biological sciences. Psychology; Lipopolysaccaharides; Medical sciences; Microbiology; Microcystins; Microcystins - analysis; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Phormidium tenue; Saudi Arabia; Statistics, Nonparametric; Toxicology; Treated waters; Water - chemistry; Water Microbiology; Water Supply
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2007.02.021

Occurrence of toxic cyanobacteria in drinking and recreational waters poses human health at risk as they can release potent toxins into the water. In the present study, open and covered treated-water storage reservoirs as well as their relevant tap waters in Abha city, Saudi Arabia, were surveyed for the presence of cyanobacteria and their toxins. The results revealed the contamination of most open reservoir and tap waters by algae and cyanobacteria, with an abundance of toxigenic species of cyanobacteria. Depending on the results of the Limulus amebocyte lysate (LAL) assay and enzyme linked immunosorbent assay (ELISA), endotoxins and microcystins (MCYSTs) were found in most open reservoir and tap waters at concentrations up to 32 EU ml −1 and 0.3 μg ml −1, respectively. The extracts of axenic cultures of most cyanobacterial species isolated from these reservoirs showed activity to LAL assay, with large endotoxin amounts obtained in Calothrix parietina (490 EU g −1) and Phormidium tenue (210 EU g −1). Based on ELISA and HPLC analysis for these extracts, only C. parietina can produce MCYSTs (202 μg g −1) with a profile consisting of MCYST-RR and -LR. This study suggests that open treated-water storage reservoirs should be covered to prevent the presence of cyanobacteria and their toxins in such drinking and recreational waters.