Regulation of Anaphylactic IgG1 Antibody Production by IL-4 and IL-10

• Published in: International Archives of Allergy and Immunology 2006-01, Vol.141 (1), p.70-78
• Main Authors: Silva, Aldacilene S., Cavalcante, Leuridan T., Faquim-Mauro, Eliana L., Macedo, Mahasti S.
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic; Aluminum Hydroxide - immunology; Animals; Antibodies, Helminth - biosynthesis; Antibodies, Helminth - blood; Antibodies, Helminth - immunology; Antibody Formation; Antigens; Antigens, Helminth - immunology; Ascaris suum; Ascaris suum - immunology; Biological and medical sciences; Cytokines; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunoglobulin G - biosynthesis; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunoglobulins; Immunology; Immunopathology; Interferon-gamma - immunology; Interleukin-10 - immunology; Interleukin-4 - immunology; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Mutant Strains; Original Paper; Ovalbumin - immunology; Passive Cutaneous Anaphylaxis - immunology; Rodents; Th1 Cells - immunology; Th2 Cells - immunology
• ISSN: 1018-2438; 1423-0097; 1365-2567
• DOI: 10.1159/000094256

Background: Different cytokines have been implicated in the regulation of isotype expression in primary and secondary antibody responses. The aim of this study was to assess the regulation of anaphylactic IgG1 and IgE antibodies by IL-4, IL-10 and IFN-γ at different time points of the antibody response against PI, an immunosuppressive fraction of Ascaris suum extract, and ovalbumin (OVA). Methods: Wild-type or cytokine-deficient C57BL/6 or BALB/c mice were immunized with PI or OVA in different adjuvants. Twenty days later, they were boosted with the respective antigen. IgG1 and IgE antibodies produced during primary and secondary responses were measured by passive cutaneous anaphylaxis. Results: PI induced low levels of anaphylactic IgG1 antibodies in the primary response and moderate levels after the antigenic booster, which were IL-4-dependent. In the absence of IL-10 and IFN-γ, PI-specific IgG1 and IgE enhanced significantly, indicating that these cytokines downregulated antibody production in primary and secondary responses. The IgG1 response to OVA in aluminium hydroxide or complete Freund’s adjuvant was IL-4-dependent in the beginning of the primary response. Later on, it became only partially regulated by IL-4 in C57BL/6 mice and IL-4-independent in Th2-prone BALB/c mice. In contrast, IgE antibodies depended exclusively upon IL-4 during the entire time course. Conclusions:These results indicate, first, that the IL-4 dependency of anaphylactic IgG1 antibody production, mainly in the secondary response, varies among mouse strains, and, second, that the nature of the antigen determines whether IL-10 and IFN-γ limit the potential to make large amounts of anaphylactic IgG1 and IgE.