Chlorsulfuron resistant transgenic tobacco as a tool for broomrape control

Broomrape (Orobanche ramosa L.) is the most important parasitic plant that infests tobacco (Nicotiana tabacum L.). Chemical treatment of the soil is not effective and crop rotation is not acceptable to solve this problem because of the long viability period of Orobanche seeds in the soil. Applicatio...

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Bibliographic Details
Published in:Transgenic research 2005-06, Vol.14 (3), p.273-278
Main Authors: Slavov, S, Valkov, V, Batchvarova, R, Atanassova, S, Alexandrova, M, Atanassov, A
Format: Article
Language:English
Subjects:
acetolactate synthase
Agrobacterium tumefaciens - genetics
ahas3R gene
Biological and medical sciences
Biotechnology
Blotting, Southern
Brassica - genetics
chemical control
chlorsulfuron
Crop rotation
Drug Resistance - genetics
Fundamental and applied biological sciences. Psychology
Gene Transfer Techniques
Genetic engineering
Genetic technics
herbicide resistance
Herbicides
Herbicides - pharmacology
Methods. Procedures. Technologies
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana tabacum
Orobanche
Orobanche - drug effects
Orobanche ramosa
pesticide application
Plants, Genetically Modified
Polymerase Chain Reaction
Sulfonamides - pharmacology
tobacco
transgenes
Transgenic animals and transgenic plants
transgenic plants
Triazines - pharmacology
weed control
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Summary:Broomrape (Orobanche ramosa L.) is the most important parasitic plant that infests tobacco (Nicotiana tabacum L.). Chemical treatment of the soil is not effective and crop rotation is not acceptable to solve this problem because of the long viability period of Orobanche seeds in the soil. Application of systemic herbicides in the field with herbicide resistant tobacco could be a successful tool for broomrape control. Several tobacco cultivars were transformed with a mutant ahas3R gene for resistance to the herbicide chlorsulfuron (Glean, DuPont). Transformed plants were selfed and the segregation of resistance was followed in the next generation. The efficiency of the herbicide was demonstrated in greenhouse and field trials. An Orobanche/tobacco growth system was used in order to prove the lethal effect of the herbicide to the attached broomrape plants.
ISSN:0962-8819
1573-9368
DOI:10.1007/s11248-004-8081-9