Genomic regions of tomato leaf curl virus DNA satellite required for replication and for satellite-mediated delivery of heterologous DNAs

Tomato leaf curl virus (TLCV) satellite DNA (sat-DNA) is a 682 nt, circular, single-stranded molecule that lacks an open reading frame (ORF) or an apparent promoter. It contains binding motifs for the TLCV replication-associated protein, but these are dispensable for replication. To identify the reg...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 2007-07, Vol.88 (7), p.2073-2077
Main Authors: Li, D, Behjatnia, S.A.A, Dry, I.B, Randles, J.W, Eini, O, Ali Rezaian, M
Format: Article
Language:English
Subjects:
Base Sequence
Begomovirus - genetics
Begomovirus - pathogenicity
Begomovirus - physiology
Biological and medical sciences
DNA, Recombinant - genetics
DNA, Satellite - genetics
DNA, Viral - genetics
DNA-binding domains
Fundamental and applied biological sciences. Psychology
Gene Silencing
Genes, Reporter
Genetic Vectors
Genome, Viral
Glucuronidase - genetics
microbial genetics
Microbiology
Miscellaneous
molecular sequence data
Mutagenesis
Nicotiana - genetics
Nicotiana - virology
Nicotiana benthamiana
open reading frames
pathogenicity
Plant Diseases - virology
Plants, Genetically Modified
promoter regions
satellite DNA
Sequence Deletion
Solanum lycopersicum - virology
Tomato leaf curl virus
viral proteins
Virology
virulence
virus replication
Virus Replication - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tomato leaf curl virus (TLCV) satellite DNA (sat-DNA) is a 682 nt, circular, single-stranded molecule that lacks an open reading frame (ORF) or an apparent promoter. It contains binding motifs for the TLCV replication-associated protein, but these are dispensable for replication. To identify the regions of the sat-DNA critical for replication, the entire sequence was scanned by deletion/replacement mutagenesis. Transient assays using Nicotiana benthamiana revealed that sequences within nt 296-35 (through nt 682) are essential for replication. Sequence deletions and replacements between nt 35 and 296 were tolerated but with a significant loss of infectivity, indicating that genome size strongly influences replication efficiency. Within the permissible region, inserts of 100-700 nt were retained in transient assays although with a slight reduction in replication. In addition, sat-DNA constructs containing short non-viral DNAs replicated and spread in tobacco plants, indicating their potential as gene-delivery vectors.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.82853-0