Partial resistance to Bean golden mosaic virus in a transgenic common bean ( Phaseolus vulgaris L.) line expressing a mutated rep gene

The rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication. A mutated rep gene with amino acid codon change in the putative nucleoside triphosphate (NTP) binding motif D262R was created. Phaseolus vulgaris transformation was achieved with a vector that contained the mutated r...

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Bibliographic Details
Published in:Plant science (Limerick) 2006-11, Vol.171 (5), p.565-571
Main Authors: Faria, Josias C., Albino, Margareth M.C., Dias, Bárbara B.A., Cançado, Letícia J., da Cunha, Nicolau B., de M. Silva, Lílian, Vianna, Giovanni R., Aragão, Francisco J.L.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Bean golden mosaic virus
beans
BGMV
Biological and medical sciences
disease resistance
Dry bean
Fundamental and applied biological sciences. Psychology
Geminivirus
Genetics and breeding of economic plants
Pest resistance
Phaseolus vulgaris
Plant pathogens
plant viruses
rep gene transdominance
replication associated protein
transgenes
transgenic plants
Varietal selection. Specialized plant breeding, plant breeding aims
viral proteins
Virus resistance
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Summary:The rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication. A mutated rep gene with amino acid codon change in the putative nucleoside triphosphate (NTP) binding motif D262R was created. Phaseolus vulgaris transformation was achieved with a vector that contained the mutated rep and bar genes. A total of 17 initial (T 0) transformants were analyzed. One line (M1/4) showed tolerance to glufosinate ammonium and partial resistance to the virus, that is, disease incidence depended on inoculation level. The incidence of BGMV increased with the increasing number of viruliferous whiteflies per plant, both in the transgenic and in the control plants. However, the number of symptomless plants was significantly higher in the transgenic group. The line M1/4 was studied during several generations and presented stability in the transgene loci and virus resistance. Southern blot analysis with genomic DNA of eight generations led to an estimate of two copies of the rep gene integrated at the same locus. RT-PCR analysis revealed the presence of both bar and rep genes transcripts. The mutated REP protein was present in amounts detectable by Western blot analysis in transgenic plants.
ISSN:0168-9452
1873-2259
DOI:10.1016/j.plantsci.2006.06.010