Attenuation of oxidative stress and artificial wound closure in C2C12 myoblasts induced by sequential extracts of Boerhavia diffusa

Objectives Whole plants of Boerhavia diffusa L. are widely used medicine in Ghana and other tropical countries, for the treatment of wounds and other ailments. The aim of the study was to determine the ability of sequential extracts of B. diffusa to influence oxidation and wound closure in myoblast...

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Bibliographic Details
Published in:Journal of pharmacy and pharmacology 2018-01, Vol.70 (1), p.111-123
Main Authors: Yahaya, Ewura Seidu, Cordier, Werner, Steenkamp, Paul Anton, Steenkamp, Vanessa
Format: Article
Language:English
Subjects:
Acetic acid
Animals
Boerhavia diffusa
Cell adhesion & migration
Cell Line
Cell migration
Closures
Cytotoxicity
Ethyl acetate
Extraction plants
Ghana
Inhibitory Concentration 50
Medicinal plants
Medicine, African Traditional
Methanol
Mice
Microscopy
Migration
Myoblasts
Myoblasts - drug effects
Myoblasts - metabolism
Nyctaginaceae - chemistry
Oxidation
Oxidative stress
Oxidative Stress - drug effects
Plant extracts
Plant Extracts - administration & dosage
Plant Extracts - pharmacology
Plant protection
Polarity
Pretreatment
Reactive oxygen species
Reactive Oxygen Species - metabolism
Solvents - chemistry
Staining
Sulforhodamine
Water treatment
Wound healing
Wound Healing - drug effects
wounds
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Summary:Objectives Whole plants of Boerhavia diffusa L. are widely used medicine in Ghana and other tropical countries, for the treatment of wounds and other ailments. The aim of the study was to determine the ability of sequential extracts of B. diffusa to influence oxidation and wound closure in myoblast cells in vitro. Methods Sequential extracts were prepared from the whole plant using four solvents of increasing polarity (hexane, ethyl acetate, methanol and water). Cytotoxicity was determined using the sulforhodamine B staining assay, phase‐contrast microscopy, plasDIC microscopy and live–dead staining. Extracts were tested for their ability to reduce 2,2′‐azobis(2‐amidinopropane) dihydrochloride (AAPH)‐induced oxidation and mediate cell migration after artificial wound generation in C2C12 myoblast cells using the scratch wound assay. Key findings All extracts indicated negligible cytotoxicity (IC50 > 100 μg/ml), and microscopic evaluation showed no difference from negative controls. AAPH induced a 2.87‐fold increase in reactive oxygen species compared to the negative control. Pretreatment with 100 μg/ml of the extracts reduced AAPH‐induced oxidation to 1.70‐fold of the untreated controls (P 
ISSN:0022-3573
2042-7158
DOI:10.1111/jphp.12833