Influence of ciprofloxacin‐based additives on the hydrolysis of nanofiber polyurethane membranes

• Published in: Journal of biomedical materials research. Part A 2018-05, Vol.106 (5), p.1211-1222
• Main Authors: Wright, Meghan E. E., Wong, Andrew T., Levitt, Daniel, Parrag, Ian C., Yang, Meilin, Santerre, J. Paul
• Format: Article
• Language: English
• Subjects: Additives; Anti-Infective Agents - pharmacology; Antibiotics; antimicrobial; Antimicrobial agents; Biomaterials; Calorimetry, Differential Scanning; Cell Line; Chemical bonds; Ciprofloxacin; Ciprofloxacin - pharmacology; Degradation; Drug delivery systems; Drug Liberation; drug release; Fibroblasts - drug effects; Gingiva - cytology; Humans; Hydrogen Bonding; Hydrolysis; Kinetics; Membranes; Membranes, Artificial; Microbial Sensitivity Tests; Minimum inhibitory concentration; Nanofibers; Nanofibers - chemistry; Periodontium; Polycarbonate; Polyurethane; Polyurethane resins; Polyurethanes - chemistry; Porphyromonas gingivalis - drug effects; Regeneration; Scaffolds; Soft tissues; Spectroscopy, Fourier Transform Infrared; Tissue engineering; Tissue Scaffolds - chemistry; Triethylene glycol; Water
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.36318

A degradable polycarbonate urethane (PCNU) and an antimicrobial oligomer (AO) were used to generate anti‐infective nanofiber scaffolds through blend electrospinning. The AO consists of two molecules of ciprofloxacin (CF) bound through hydrolysable linkages to triethylene glycol. The membranes were conceived for use as tissue engineering scaffolds for the regeneration of soft tissues for the periodontium, where there would be a need for a local dose of antibiotic to the periodontal space as the scaffold degrades in order to prevent biomaterial‐associated infection. Scaffolds were made using AO at 7 and 15% w/w equivalent CF, and compared to scaffolds with 15% w/w CF (with HCl counterion). AO was hydrolyzed and released CF continuously over 28 days, while the 15% w/w CF HCl scaffolds showed a burst release within hours, with no subsequent release in the subsequent 28 day period. Released CF from both the AO and CF HCl scaffolds had a similar minimum inhibitory concentration to that of off‐the‐shelf CF. Interestingly, the introduction of drug in either form (AO or CF HCl) was found to increase the hydrolytic stability of the electrospun degradable PCNU scaffold matrix itself. The alteration of hydrolysis kinetics was attributed to changes in the hydrogen bonding character and microstructure within the scaffolds, introduced by the presence of CF. This study has revealed that in generating in situ drug release systems, the secondary effects of the added drug on the degradation properties of the polymeric carriers must be considered, particularly for systems that act dually as tissue engineering scaffolds. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1211–1222, 2018.