Cynaropicrin, a sesquiterpene lactone, as a new strong regulator of CD29 and CD98 functions

• Published in: Biochemical and biophysical research communications 2004-01, Vol.313 (4), p.954-961
• Main Authors: Cho, Jae Youl, Kim, Ae Ra, Joo, Hong-Gu, Kim, Byung-hun, Rhee, Man Hee, Yoo, Eun Sook, Katz, David R, Chain, Benjamin M, Jung, Jee H
• Format: Article
• Language: English
• Subjects: Adhesion molecule; Antigens, CD; CD147; CD29 (β1 integrins); CD98; Cell Aggregation - drug effects; Cynaropicrin; ERK; Fusion Regulatory Protein-1 - metabolism; Homotypic aggregation; Humans; Integrin beta1 - metabolism; Lactones - chemistry; Lactones - pharmacology; Leukosialin; Macrophages - cytology; Macrophages - drug effects; Macrophages - immunology; Macrophages - metabolism; MAP Kinase Signaling System - drug effects; Mitogen-Activated Protein Kinases - metabolism; Phosphorylation; Sesquiterpene lactone; Sesquiterpenes - chemistry; Sesquiterpenes - pharmacology; Sialoglycoproteins - metabolism; Tyrosine - metabolism; U937 Cells
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2003.12.026

Cynaropicrin is a sesquiterpene lactone displaying immunomodulatory effects on the production of cytokine and nitric oxide from macrophages/monocytes. In this study we have examined inhibitory effect of cynaropicrin on activation of major adhesion molecules [CD29 (β1 integrins), CD43, and CD98] on the cells assessed by U937 (promonocytic cells) homotypic aggregation. Cynaropicrin potently blocked CD29 (β1 integrins)- and CD98-induced homotypic aggregation with IC 50 values of 3.46 and 2.98 μM, respectively, without displaying cytotoxicity. Similarly, flow cytometric analysis exhibited that cynaropicrin down-regulated strikingly surface level of CD29 and CD147, a functional regulator of CD98, but not CD43. More importantly, cynaropicrin inhibition was linked to blockade of extracellular signal-related kinase (ERK) activation and distinct from other enzyme inhibitors including rottlerin, propranolol, forskolin, and chloroquine, but not cytochalasin B. Therefore, our finding is the first demonstration that cynaropicrin may be a potent functional regulator of CD29 and CD98 via interrupting ERK activation which may be linked to cytoskeleton rearrangement, suggesting further application to CD29- and CD98-mediated diseases such as virus-induced chronic inflammation, and invasion, migration, and metastasis of leukocyte cancer cells.