Microbial degradation of chlorobenzene under oxygen-limited conditions leads to accumulation of 3-chlorocatechol

Five bacterial strains (Acidovorax facilis B517, Cellulomonas turbata B529, Pseudomonas veronii B547, Pseudomonas veronii B549, and Paenibacillus polymyxa B550) isolated on chlorobenzene as the sole source of carbon and energy were screened for the accumulation of the putative metabolic intermediate...

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Bibliographic Details
Published in:Environmental toxicology and chemistry 2004-02, Vol.23 (2), p.265-270
Main Authors: Vogt, Carsten, Simon, Dieter, Alfreider, Albin, Babel, Wolfgang
Format: Article
Language:English
Subjects:
3-Chlorocatechol
Acidovorax
Acidovorax facilis
Animal, plant and microbial ecology
Applied ecology
Aquifer
Bacteria - growth & development
Bacteria - metabolism
Biodegradation of pollutants
Biodegradation, Environmental
Biological and medical sciences
Biotechnology
Catechol 1,2-Dioxygenase
Catechols - metabolism
Cellulomonas
Cellulomonas turbata
Chlorobenzene
Chlorobenzenes - metabolism
Chromatography, Gas
Dioxygenases
Ecotoxicology, biological effects of pollution
Environment and pollution
Fundamental and applied biological sciences. Psychology
General aspects
In situ bioremediation
Industrial applications and implications. Economical aspects
Oxygen - metabolism
Oxygen limitation
Oxygenases - metabolism
Paenibacillus polymyxa
Pseudomonas veronii
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Summary:Five bacterial strains (Acidovorax facilis B517, Cellulomonas turbata B529, Pseudomonas veronii B547, Pseudomonas veronii B549, and Paenibacillus polymyxa B550) isolated on chlorobenzene as the sole source of carbon and energy were screened for the accumulation of the putative metabolic intermediate 3‐chlorocatechol during growth on chlorobenzene under oxygen‐limited conditions in the presence and absence of nitrate (1 mM). 3‐Chlorocatechol accumulated in the growth media of all five strains, but accumulation was significantly less in cultures of A. facilis B517 compared to the other four strains. The presence of nitrate did not influence the biological conversion pattern. However, biologically produced nitrite reacted with 3‐chlorocatechol chemically, a reaction that masked the accumulation of 3‐chlorocatechol. For P. veronii B549, a clear relationship between the presence of 3‐chlorocatechol in the medium and low oxygen concentrations was demonstrated. The assumption is made that accumulation of 3‐chlorocatechol is due to the low enzymatic turnover of the 3‐chlorocatechol cleaving enzyme, catechol‐1,2‐dioxygenase, at low oxygen concentrations.
ISSN:0730-7268
1552-8618
DOI:10.1897/02-446