Simultaneous quantification of fluoxetine and norfluoxetine in colostrum and mature human milk using a 2-dimensional liquid chromatography–tandem mass spectrometry system

•Drugs in human milk a greener approach.•On-flow depletion of milk proteins.•A 2D LC–MS/MS protocol for quantification of fluoxetine and norfluxetine in breast milk and colostrum. A two-dimensional liquid chromatography system coupled to triple quadrupole tandem mass spectrometer (2D LC–MS/MS) was e...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2018-02, Vol.150, p.362-367
Main Authors: Lopes, Bianca Rebelo, Cassiano, Neila Maria, Carvalho, Daniela Miarelli, Moisés, Elaine Christine Dantas, Cass, Quezia Bezerra
Format: Article
Language:English
Subjects:
Breast milk
Calibration
Chromatography, Liquid - methods
Chromatography, Liquid - standards
Colostrum - chemistry
Female
Fluoxetine
Fluoxetine - analogs & derivatives
Fluoxetine - analysis
Humans
Limit of Detection
Linear Models
Milk, Human - chemistry
Norfluoxetine
Reference Standards
Reproducibility of Results
Restricted access media
Tandem Mass Spectrometry - standards
Two-dimensional liquid chromatography
Workflow
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Summary:•Drugs in human milk a greener approach.•On-flow depletion of milk proteins.•A 2D LC–MS/MS protocol for quantification of fluoxetine and norfluxetine in breast milk and colostrum. A two-dimensional liquid chromatography system coupled to triple quadrupole tandem mass spectrometer (2D LC–MS/MS) was employed for the determination of fluoxetine (FLU) and norfluoxetine (N-FLU) in colostrum and mature milk by direct sample injection. With a run time of 12 min representing a gain in throughput analysis, the validated methods furnished selectivity, extraction efficiency, accuracy, and precision in accordance with the criteria preconized by the European Medicines Agency guidelines. With a linear range of 3.00–150 ng/mL for FLU and 4.00–200 ng/mL for N-FLU they were applied to the analysis of colostrum and mature milk samples from nursing mothers. The paper discusses the differences and similarity of sample preparation for this two sample matrices. The herein reported methods are an advance in sample preparation procedures providing waste reduction and a sustainable approach.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2017.12.041