Successful Gene Transfer into Dendritic Cells with Cationized Gelatin and Plasmid DNA Complexes Via a Phagocytosis-dependent Mechanism

The use of gene-modified dendritic cells (DC) is a powerful tool to enhance antitumor immune responses stimulated by these cells in cancer immunotherapy. Cationized gelatin is preferably incorporated via phagocytosis and is gradually degraded by proteolysis while buffering lysosomal activity. This m...

Full description

Saved in:
Bibliographic Details
Published in:Anticancer research 2006-05, Vol.26 (3A), p.1957-1963
Main Authors: INADA, Satoshi, FUJIWARA, Hitoshi, ATSUJI, Kiyoto, TAKASHIMA, Kazuhiro, ARAKI, Yasunobu, KUBOTA, Takeshi, TABATA, Yasuhiko, YAMAGISHI, Hisakazu
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Dendritic Cells - cytology
Dendritic Cells - immunology
Dendritic Cells - physiology
Gelatin - metabolism
Green Fluorescent Proteins - genetics
Humans
Interleukin-12 - genetics
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - immunology
Leukocytes, Mononuclear - physiology
Medical sciences
Mice
Phagocytosis
Plasmids - genetics
Plasmids - metabolism
Transfection - methods
Tumors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The use of gene-modified dendritic cells (DC) is a powerful tool to enhance antitumor immune responses stimulated by these cells in cancer immunotherapy. Cationized gelatin is preferably incorporated via phagocytosis and is gradually degraded by proteolysis while buffering lysosomal activity. This may be appropriate for gene transfer into phagocytic cells, such as immature DC. In the present study, successful transfection into monocyte-derived immature DC was demonstrated using cationized gelatin and plasmid DNA complexes. A high transfection efficiency, approaching 16%, was obtained upon transfection of the enhanced green fluorescent protein (EGFP) gene as evaluated by flow cytometry. Transgene expression of EGFP and murine interleukin 12 were also detected by RT-PCR. The antigen-presenting capacity of the transfected DC was equal to that of untransfected DC as evaluated by the allogeneic mixed lymphocyte reaction. Cationized gelatin has the potential to be a unique non-viral vector for gene transfer into DC.
ISSN:0250-7005
1791-7530