Cooperative coupling and role of heme a in the proton pump of heme-copper oxidases

• Published in: Biochimie 1998-10, Vol.80 (10), p.821-836
• Main Authors: Papa, Sergio, Capitanio, Nazzareno, Villani, Gaetano, Capitanio, Giuseppe, Bizzoca, Antonella, Palese, Luigi L., Carlino, Valeria, De Nitto, Emanuele
• Format: Article
• Language: English
• Subjects: Animals; Bacillus subtilis; Cattle; cooperative coupling; Copper - metabolism; DNA Mutational Analysis; Electron Transport Complex IV - metabolism; Heme - analogs & derivatives; Heme - physiology; heme-copper oxidases; Hydrogen-Ion Concentration; Liver - enzymology; Mitochondria - enzymology; Models, Chemical; Models, Molecular; Myocardium - enzymology; Oxidation-Reduction; proton pump; Proton Pumps - chemistry; Rats
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/S0300-9084(00)88877-X

In the last few years, evidence has accumulated supporting the applicability of the cooperative model of proton pumps in cytochrome systems, vectorial Bohr mechanism, to heme-copper oxidases. The vectorial Bohr mechanism is based on short- and long-range protonmotive cooperative effects linked to redox transitions of the metal centers. The crystal structure of oxidized and reduced bovine-heart cytochrome c oxidase reveals, upon reduction, the occurrence of long-range conformational changes in subunit 1 of the oxidase. Analysis of the crystal structure of cytochrome c oxidase shows the existence of hydrogen-bonded networks of amino acid residues which could undergo redox-linked pK shifts resulting in transmembrane proton translocation. Our group has identified four proteolytic groups undergoing reversible redox-linked pK shifts. Two groups result in being linked to redox transitions of heme a 3. One group is apparently linked to Cu B. The fourth group is linked to oxido-reduction of heme a. We have shown that the proton transfer resulting from the redox Bohr effects linked to heme a and Cu B in the bovine oxidase displays membrane vectorial asymmetry, i.e., protons are taken up from the inner aqueous space (N), upon reduction, and released in the external space (P), upon oxidation of the metals. This direction of proton uptake and release is just what is expected from the vectorial Bohr mechanism. The group linked to heme a, which can transfer up to 0.9 H +/e at pHs around neutrality, can provide the major contribution to the proton pump. It is proposed that translocation of pumped protons, linked to electron flow through heme a, utilizes a channel (channel D) which extends from a conserved aspartate at the N entrance to a conserved glutamate located between heme a and the binuclear center. The carboxylic group of this glutamic acid, after having delivered, upon electron flow through heme a, pumped protons towards the P phase, once reprotonated from the N phase, moves to deliver, subsequently, to the binuclear center chemical protons consumed in the conservation of the peroxy to ferryl and of the latter to the oxy intermediate in the redox cycle. Site-directed mutagenesis of protolytic residues in subunit 1 of the aa 3-600 quinol oxidase of Bacillus subtilis to non-polar residues revealed that the conserved Lys 304 is critical for the proton pumping activity of the oxidase. Crystal structures of cytochrome c oxidase show that this lysine is at the N entranc