Production of Homogeneous and Viable Verticillium dahliae Microsclerotia Effective for Verticillium Wilt Studies

Artificial production of Verticillium dahliae microsclerotia using potato dextrose agar plates covered by a cellophane sheet was optimized regarding thickness and sterilization method of the film. The objective was to standardize production per plate, homogeneity (size and propagules individuality)...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology (Faisalābād, Pakistan) Pakistan), 2005-01, Vol.4 (4), p.421-428
Main Authors: Lopez-Escudero, F J, Mwanza, C, Blanco-Lopez, MA
Format: Article
Language:English
Subjects:
Solanum tuberosum
Verticillium
Verticillium dahliae
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Artificial production of Verticillium dahliae microsclerotia using potato dextrose agar plates covered by a cellophane sheet was optimized regarding thickness and sterilization method of the film. The objective was to standardize production per plate, homogeneity (size and propagules individuality) and viability (germinability and infective capability) of inoculum, for epidemiological studies. Autoclaved cellophane film, 600 urn in thickness, yielded the highest production of microsclerotia, after 7-10 days of incubation at 22 degree C, without causing its degradation. Amount of microsclerotia per plate was significantly higher for the defoliating (331.9x10 super(3)) than the non-defoliating (202.1x10 super(3)) isolate of the pathogen, maintaining the 93.0 and 89.6% of germinability, respectively, after 1 year. Disease developed on cotton plants of cultivars Tabladilla-16 and Tauro, grown on infested soils with 0.5 to 125 microsclerotia per gram of soil, demonstrated its capability of survival and infection. A high relationship between inoculum density and onset and severity symptoms was observed, that depended on the isolate virulence and cultivar susceptibility.
ISSN:1682-296X