Degradation of trichothecene mycotoxins by chicken intestinal microbes

The degradation of 12 trichothecene mycotoxins by chicken intestinal microbes was monitored by liquid chromatography–ultraviolet-mass spectrometry under positive ion atmospheric pressure chemical ionization. Two pathways were observed: deacylation and deepoxidation. Essentially complete conversions...

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Bibliographic Details
Published in:Food and chemical toxicology 2007, Vol.45 (1), p.136-143
Main Authors: Young, J. Christopher, Zhou, Ting, Yu, Hai, Zhu, Honghui, Gong, Jianhua
Format: Article
Language:English
Subjects:
acetylation
Animals
Bacteria - metabolism
biodegradation
Biological and medical sciences
Biotransformation
chemical ionization
Chicken intestinal microbes
Chickens
Chromatography, High Pressure Liquid
Deacetylation
Deepoxidation
Degradation
feed contamination
feeds
intestinal microorganisms
Intestines - microbiology
ionization
Medical sciences
metabolic detoxification
microbial activity
Mycotoxins
Plant poisons toxicology
Poisons - analysis
Poisons - metabolism
poultry feeding
Spectrometry, Mass, Electrospray Ionization
toxic substances
Toxicology
Trichothecenes
Trichothecenes - analysis
Trichothecenes - metabolism
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Summary:The degradation of 12 trichothecene mycotoxins by chicken intestinal microbes was monitored by liquid chromatography–ultraviolet-mass spectrometry under positive ion atmospheric pressure chemical ionization. Two pathways were observed: deacylation and deepoxidation. Essentially complete conversions to the deepoxy metabolites were observed for the non-acylated trichothecenes 4-deoxynivalenol, nivalenol, and verrucarol. However, deacetylation was the predominant pathway for the monoacetyl trichothecenes 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol (15ADON), and fusarenon X. Small amounts of the deepoxy metabolites were observed from 15ADON and large amounts from 15-monoacetoxyscirpenol where steric hindrance protected the C-15 acetyl groups from enzymatic attack. Diacetylated trichothecenes diacetoxyscirpenol and neosolaniol exhibited only deacetylation. The larger isovaleryl functionality was resistant to removal and deepoxidation was the prevalent reaction in HT-2 toxin and T-2 triol, whereas T2 toxin showed only deacetylation.
ISSN:0278-6915
1873-6351
DOI:10.1016/j.fct.2006.07.028