Transcriptional regulation of chickpea ferritin CaFer1 influences its role in iron homeostasis and stress response

Ferritin, ubiquitous among all living organisms except yeast, exhibits iron-regulated expression. In plants, this regulation is applied through transcriptional control. Previous studies established the presence of two types of cis-acting elements in the promoter region: the iron regulatory element (...

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Bibliographic Details
Published in:Journal of plant physiology 2018-03, Vol.222, p.9-16
Main Authors: Parveen, Shaista, Pandey, Aarti, Jameel, Neha, Chakraborty, Subhra, Chakraborty, Niranjan
Format: Article
Language:English
Subjects:
Binding sites
cis- and trans-acting elements
Corn
Electrophoretic mobility
Environmental conditions
Ferritin
Gene expression
Gene regulation
Homeostasis
Iron
Iron deficiency
Iron-responsive genes
Nutrient deficiency
Oxidative stress
Phylogenetic relationship
Phylogenetics
Phylogeny
Phytoferritin
Protein-protein interaction
Regulatory sequences
Soybeans
Studies
Transcriptional regulation
Yeast
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Summary:Ferritin, ubiquitous among all living organisms except yeast, exhibits iron-regulated expression. In plants, this regulation is applied through transcriptional control. Previous studies established the presence of two types of cis-acting elements in the promoter region: the iron regulatory element (FRE) in soybean and the iron-dependent regulatory sequence (IDRS) in maize and Arabidopsis. Adverse environmental conditions (e.g. water-deficit and oxidative stress) are known to modulate the expression of phytoferritin genes. In this study, we cloned and investigated the promoter sequence of a chickpea ferritin, designated CaFer1. Phylogenetic analysis of the CaFer1 promoter revealed its evolutionary relationship with other phytoferritins. The CaFer1 promoter exhibited several putative regulatory elements including two known transcription factor (TF) binding sites, Athb-1 and Myb.Ph. Electrophoretic mobility shift assay confirmed the sequence-specific binding of Athb-1 and Myb.Ph on the CaFer1 promoter. The TF-binding dynamics of CaFer1 showed high induction under conditions of iron-deficiency and water-deficit. We also demonstrated the possible interaction of CaFer1 with IRT1, a key component of the iron uptake system in plants, indicating its involvement in maintaining cellular iron levels. These results provide new insights into the underlying mechanisms of function of these interacting factors in CaFer1-mediated iron homeostasis and the stress response in plants.
ISSN:0176-1617
1618-1328
DOI:10.1016/j.jplph.2017.12.015