The protective effects of vitamin C on apoptosis, DNA damage and proteome of pufferfish (Takifugu obscurus) under low temperature stress

The aim of this study was to investigate the protective effects of vitamin C on apoptosis, DNA damage and proteome of pufferfish under low temperature stress. Six diets were formulated to contain 2.60, 48.90, 95.50, 189.83, 382.40, 779.53mg/kg vitamin C. After 8-week feeding trial, fish were exposed...

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Bibliographic Details
Published in:Journal of thermal biology 2018-01, Vol.71, p.128-135
Main Authors: Cheng, Chang-Hong, Liang, Hai-Yan, Luo, Sheng-Wei, Wang, An-Li, Ye, Chao-Xia
Format: Article
Language:English
Subjects:
Apoptosis
Ascorbic acid
Calcium
Cold stress
Comparative proteomics
Cytoskeleton
Diet
DNA damage
Energy metabolism
Fish
Immune response
Nutrient deficiency
Proteomics
Stress response
Takifugu obscurus
Temperature
Temperature effects
Vitamin C
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Summary:The aim of this study was to investigate the protective effects of vitamin C on apoptosis, DNA damage and proteome of pufferfish under low temperature stress. Six diets were formulated to contain 2.60, 48.90, 95.50, 189.83, 382.40, 779.53mg/kg vitamin C. After 8-week feeding trial, fish were exposed to low temperature challenge. The results showed that pufferfish receiving vitamin C diet exhibited a significant decrease in ROS production (48.9–189.83mg/kg vitamin C diet groups), cytoplasmic free-Ca2+ concentration (48.9–779.53mg/kg vitamin C diet groups), apoptotic cell ratio (95.5–779.53mg/kg vitamin C diet groups) and DNA damage (189.83–779.53mg/kg vitamin C diet groups) under low temperature stress in comparison with those of control. We also investigated the alteration in protein expression under low temperature stress by a comparative proteomic analysis. The results demonstrated that 24 protein spots showed significantly differential expression in the cold-stress-treated group compared with those of the control group, and 5 protein spots were successfully identified. Furthermore, comparative proteomic analysis revealed that vitamin C could increase expressed proteins related to energy metabolism, immune responses and cytoskeleton. These findings would be helpful to understand the protective effects of vitamin C against cold stress. •Low temperature induced ROS, apoptosis and DNA damage.•Vitamin C could C enhance resistance against cold stress.•Comparative proteomic was established.•Potential impacts of vitamin C on improvement of low temperature stress are discussed.
ISSN:0306-4565
1879-0992
DOI:10.1016/j.jtherbio.2017.11.004