A New Fluorogenic Probe for the Detection of endo‐β‐N‐Acetylglucosaminidase

• Published in: Chembiochem : a European journal of chemical biology 2018-04, Vol.19 (7), p.660-663
• Main Authors: Ishii, Nozomi, Sunaga, Chie, Sano, Kanae, Huang, Chengcheng, Iino, Kenta, Matsuzaki, Yuji, Suzuki, Tadashi, Matsuo, Ichiro
• Format: Article
• Language: English
• Subjects: Acetylglucosaminidase - chemistry; Aniline Compounds - chemical synthesis; Aniline Compounds - chemistry; Animals; carbohydrates; Chemical compounds; endoglycosidases; Enzyme Assays - methods; Fluorescence; Fluorescence Resonance Energy Transfer; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; fluorescent probes; glycoconjugates; Humans; hydrolases; Hydrolysis; Inhibitors; Mice; Oligosaccharides - chemical synthesis; Oligosaccharides - chemistry; ortho-Aminobenzoates - chemical synthesis; ortho-Aminobenzoates - chemistry; ortho-Aminobenzoates - radiation effects; Pharmacology; Ultraviolet Rays
• ISSN: 1439-4227; 1439-7633
• DOI: 10.1002/cbic.201700662

We developed a fluorescence‐quenching‐based assay system to determine the hydrolysis activity of endo‐β‐N‐acetylglucosaminidases (ENGases). The pentasaccharide derivative 1 was labeled with an N‐methylanthraniloyl group as a reporter dye at the non‐reducing end and with a 2,4‐dinitrophenyl group as a quencher molecule at the reducing end. This derivative is hydrolyzed by ENGase, resulting in an increase in fluorescence intensity. Thus, the fluorescence signal is directly proportional to the amount of the tetrasaccharide derivative, hence allowing ENGase activity to be evaluated easily and quantitatively. Using this system, we succeeded in measuring the hydrolysis activities of ENGases and thus the inhibitory activities of known inhibitors. We confirmed that this assay system is suitable for high‐throughput screening for potential inhibitors of human ENGase that might serve as therapeutic agents for the treatment of N‐glycanase 1 (NGLY1) deficiency. Real‐time monitoring of ENGase activity: A fluorescence‐quenching‐based assay system to determine the hydrolysis activity of endo‐β‐N‐acetylglucosaminidase (ENGase) was developed. We succeeded in measuring the hydrolysis activities of ENGases quantitatively. This assay system is suitable for screening potential inhibitors of human ENGase, which could serve as therapeutic agents for N‐glycanase 1 (NGLY1) deficiency.