Mycotoxin production of Alternaria strains isolated from Korean barley grains determined by LC-MS/MS

Twenty-four Alternaria strains were isolated from barley grain samples. These strains were screened for the production of mycotoxins on rice medium using thin layer chromatography. All 24 strains produced at least one of the five mycotoxins (ALT, AOH, ATX-I, AME, and TeA). Three representative strai...

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Bibliographic Details
Published in:International journal of food microbiology 2018-03, Vol.268, p.44-52
Main Authors: Nguyen, Thuong T.T., Kim, Jueun, Jeon, Sun Jeong, Lee, Chul Won, Magan, Naresh, Lee, Hyang Burm
Format: Article
Language:English
Subjects:
Agricultural production
Alternaria - isolation & purification
Alternaria - metabolism
Alternaria mycotoxins
Alternaria strains
Analysis conditions
Barley
Chromatography, Liquid
Chromatography, Thin Layer
Determination
Edible Grain - microbiology
Food Contamination - analysis
Fungi
Grain
Hordeum - microbiology
Lactones - analysis
LC–MS/MS
Mass spectrometry
Mass spectroscopy
Mycotoxins
Mycotoxins - analysis
Mycotoxins - biosynthesis
Pathogens
Republic of Korea
Sample preparation
Simultaneous quantification
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Tea
Thin layer chromatography
Toxins
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Summary:Twenty-four Alternaria strains were isolated from barley grain samples. These strains were screened for the production of mycotoxins on rice medium using thin layer chromatography. All 24 strains produced at least one of the five mycotoxins (ALT, AOH, ATX-I, AME, and TeA). Three representative strains, namely EML-BLDF1-4, EML-BLDF1-14, and EML-BLDF1-18, were further analyzed using a new LC–MS/MS-based mycotoxin quantification method. This method was used to detect and quantify Alternaria mycotoxins. We used positive ion electrospray mass spectrometry with multiple reaction mode (MRM) for the simultaneous quantification of various Alternaria mycotoxins produced by these strains. Five Alternaria toxins (ALT, ATX-I, AOH, AME, and TeA) were detected and quantified. Sample preparation included methanol extraction, concentration, and injection into LC–MS/MS. Limit of detection ranged from 0.13 to 4μg/mL and limit of quantification ranged from 0.25 to 8μg/mL. •All Alternaria strains isolated from Korean barley grains produced mycotoxins in rice kernel medium.•Five Alternaria mycotoxins in rice medium was simultaneously determined by LC/MS/MS.•The LOD and LOQ of mycotoxins ranged from 0.13 to 4μg/mL and 0.25 to 8μg/mL.
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2018.01.003