A profilin gene promoter from switchgrass (Panicum virgatum L.) directs strong and specific transgene expression to vascular bundles in rice

• Published in: Plant cell reports 2018-04, Vol.37 (4), p.587-597
• Main Authors: Xu, Wenzhi, Liu, Wusheng, Ye, Rongjian, Mazarei, Mitra, Huang, Debao, Zhang, Xinquan, Stewart, C. Neal
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Biomedical and Life Sciences; Biotechnology; Cell Biology; Cell walls; Energy crops; Flowers; Flowers - genetics; Flowers - metabolism; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene silencing; Genetic engineering; Glucuronidase - genetics; Glucuronidase - metabolism; Leaves; Life Sciences; Original Article; Oryza - genetics; Oryza - metabolism; Oryza sativa; Panicum - genetics; Panicum virgatum; Phloem; Plant Biochemistry; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Roots - genetics; Plant Roots - metabolism; Plant Sciences; Plant tissues; Plant Vascular Bundle - genetics; Plant Vascular Bundle - metabolism; Plants, Genetically Modified; Profilin; Profilins - genetics; Promoter Regions, Genetic - genetics; Promoters; Renewable energy; Rice; Sequence Homology, Amino Acid; Sheaths; Transgenes; Transgenes - genetics; Vascular system (plant anatomy); Vascular tissue; Xylem
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-018-2253-1

Key message A switchgrass vascular tissue-specific promoter ( PvPfn2 ) and its 5′-end serial deletions drive high levels of vascular bundle transgene expression in transgenic rice. Constitutive promoters are widely used for crop genetic engineering, which can result in multiple off-target effects, including suboptimal growth and epigenetic gene silencing. These problems can be potentially avoided using tissue-specific promoters for targeted transgene expression. One particularly urgent need for targeted cell wall modification in bioenergy crops, such as switchgrass ( Panicum virgatum L.), is the development of vasculature-active promoters to express cell wall-affective genes only in the specific tissues, i.e., xylem and phloem. From a switchgrass expression atlas we identified promoter sequence upstream of a vasculature-specific switchgrass profilin gene ( PvPfn2 ), especially in roots, nodes and inflorescences. When the putative full-length (1715 bp) and 5′-end serial deletions of the PvPfn2 promoter (shortest was 413 bp) were used to drive the GUS reporter expression in stably transformed rice ( Oryza sativa L.), strong vasculature-specificity was observed in various tissues including leaves, leaf sheaths, stems, and flowers. The promoters were active in both phloem and xylem. It is interesting to note that the promoter was active in many more tissues in the heterologous rice system than in switchgrass. Surprisingly, all four 5′-end promoter deletions, including the shortest fragment, had the same expression patterns as the full-length promoter and with no attenuation in GUS expression in rice. These results indicated that the PvPfn2 promoter variants are new tools to direct transgene expression specifically to vascular tissues in monocots. Of special interest is the very compact version of the promoter, which could be of use for vasculature-specific genetic engineering in monocots.