Type‐specific persistence and clearance rates of HPV genotypes in the oral and oropharyngeal mucosa in an HIV/AIDS cohort

• Published in: Journal of oral pathology & medicine 2018-04, Vol.47 (4), p.396-402
• Main Authors: Castillejos‐García, Itzel, Ramírez‐Amador, Velia A., Carrillo‐García, Adela, García‐Carrancá, Alejandro, Lizano, Marcela, Anaya‐Saavedra, Gabriela
• Format: Article
• Language: English
• Subjects: Acquired immune deficiency syndrome; Acquired Immunodeficiency Syndrome - complications; Acquired Immunodeficiency Syndrome - virology; Adult; AIDS; Cancer; Dentistry; Female; Genotype; Genotypes; Head & neck cancer; Highly active antiretroviral therapy; HIV; HIV Infections - complications; HIV Infections - virology; HIV/AIDS; Human immunodeficiency virus; Human papillomavirus; Humans; L1 gene; Longitudinal Studies; Male; Mouth Mucosa - virology; Mucosa; Mucous Membrane - virology; Nucleotide sequence; Oral cancer; Oropharynx - virology; Papillomaviridae - genetics; persistence; Primers; Risk factors; tobacco
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1111/jop.12687

Background Oral high‐risk human papillomavirus (HR‐HPV) infections are frequent and persistent among the HIV‐positive population and are associated with an increased risk for head and neck cancer (HNC). In this study, we sought to determine the incidence, persistence and clearance of HPV infections in oral and oropharyngeal samples from HIV/AIDS subjects. Methods A longitudinal, observational and analytical study was performed with an ongoing cohort of HIV/AIDS subjects in Mexico City (September 2013‐February 2015). The study was approved by institutional committees, and demographic and clinical data were registered. At the baseline and three‐month visits, oral examinations and cytobrush samples were obtained. DNA was purified, quantified and used to detect an HPV‐L1 gene fragment by nested PCR, using MY09/MY11 and GP5 + /GP6 + primers. HPV DNA products were purified, sequenced and typed according to HPV databases. Risk factors were assessed, and a multivariate modelling approach was used to determine independent effects. Results This study included 97 HIV/AIDS individuals (91% men [86.4% of which are men who have sex with men], median age: 36 years, 72.2% under HAART). From the baseline visit, HPV was observed in 55.7% (HR‐HPV: 26.8%; HPV‐18: 24.1%), with a higher HPV‐positive samples for smokers (61.1 vs 32.6%, P = .005). The three‐month overall HPV incidence was 33.9%; type‐specific HPV persistence was 33.3% (HR‐HPV: 13.3%); and 13 of the 33 (39.4%) baseline HPV‐positive individuals cleared the infection (HR‐HPV: 53.8%). Conclusions Although HR‐HPV persistence was low, and clearance of the infection was observed in most cases, a close follow‐up is necessary, given the increase in HNC among HIV‐subjects, particularly HPV‐related cancer.