Increasing Analytical Separation and Duty Cycle with Nonlinear Analytical Mobility Scan Functions in TIMS-FT-ICR MS

In this work, nonlinear, stepping analytical mobility scan functions are implemented to increase the analytical separation and duty cycle during tandem Trapped Ion Mobility Spectrometry and FT-ICR MS operation. The differences between linear and stepping scan functions are described based on length...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2018-02, Vol.90 (4), p.2446-2450
Main Authors: Benigni, Paolo, Porter, Jacob, Ridgeway, Mark. E, Park, Melvin. A, Fernandez-Lima, Francisco
Format: Article
Language:English
Subjects:
Breast cancer
Chemistry
Ionic mobility
Mathematical analysis
Medical imaging
Mobility
Nonlinear analysis
Peptides
Resolution
Sensitivity
Separation
Spectrometry
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Summary:In this work, nonlinear, stepping analytical mobility scan functions are implemented to increase the analytical separation and duty cycle during tandem Trapped Ion Mobility Spectrometry and FT-ICR MS operation. The differences between linear and stepping scan functions are described based on length of analysis, mobility scan rate, signal-to-noise, and mobility resolving power. Results showed that for the linear mobility scan function only a small fraction of the scan is sampled, resulting in the lowest duty cycle 0.5% and longest experiment times. Implementing nonlinear targeted scan functions for analysis of known mobilities resulted in increased duty cycle (0.85%) and resolving powers (R up to 300) with a 6-fold reduction in time from 30 to 5 min. For broad range characterization, a nonlinear mobility stepping scan function provided the best sensitivity, resolving power, duty cycle (4%), and points per peak. The applicability of nonlinear mobility scan functions for the analysis of complex mixtures is illustrated for the case of a direct infusion of a MCF-7 breast cancer cell digest, where isobaric peptides (e.g., DFTPAELR and TTILQSTGK) were separated in the mobility domain (R IMS: 110) and identified based on their CCS, accurate mass (R MS: 550k), and tandem MS using IRMPD in the ICR cell.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.7b04053