Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System

Transcription activator-like effector nucleases （TALENs） and clustered regularly interspaced short palindromic repeats （CRISPR）/ CRISPR-associated （Cas） systems have emerged as powerful tools for genome editing in a variety of species. Here, we report, for the first time, targeted mutagenesis in Zea...

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Bibliographic Details
Published in:Journal of genetics and genomics 2014-02, Vol.41 (2), p.63-68
Main Authors: Liang, Zhen, Zhang, Kang, Chen, Kunling, Gao, Caixia
Format: Article
Language:English
Subjects:
Base Sequence
CAS系统
Clustered Regularly Interspaced Short Palindromic Repeats - genetics
corn
CRISPR-Cas Systems - genetics
CRISPR/Cas system
DNA Restriction Enzymes - metabolism
genes
Genes, Plant - genetics
Genetic Engineering - methods
Genomics
Knock-out
Mutagenesis, Site-Directed
Mutation
nucleases
Plants, Genetically Modified
protoplasts
site-directed mutagenesis
somatic mutation
TAL-effector nucleases
transcription (genetics)
transgenic plants
Zea mays
Zea mays - genetics
体细胞突变
原生质体
基因组功能
塔
玉米
诱变
转基因植物
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