Engineering Escherichia coli for poly-(3-hydroxybutyrate) production guided by genome-scale metabolic network analysis

•Genome-scale metabolic network analysis revealed that the introduction of NOG-pathway can greatly improve P3HB yield.•The yield increased from 0.16 to 0.24g P3HB/g glucose by introducing and enhancing the NOG pathway in E. coli.•Further engineering to enhance NADPH supply and down-regulate TCA cycl...

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Bibliographic Details
Published in:Enzyme and microbial technology 2017-11, Vol.106, p.60-66
Main Authors: Zheng, Yangyang, Yuan, Qianqian, Yang, Xiaoyan, Ma, Hongwu
Format: Article
Language:English
Subjects:
acetyl coenzyme A
biodegradability
Biodegradable Plastics - chemistry
Biodegradable Plastics - metabolism
carbon
Citric Acid Cycle
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Gene Knockout Techniques
Genome, Bacterial
Genome-scale metabolic network analysis
glucose
Glucose - metabolism
glycolysis
Hydroxybutyrates - chemistry
Hydroxybutyrates - metabolism
Metabolic Engineering - methods
Metabolic Networks and Pathways - genetics
NADP (coenzyme)
Non-oxidative glycolysis pathway
phosphoketolase
plastics
Poly-(3-hydroxybutyrate)
Polyesters - chemistry
Polyesters - metabolism
tricarboxylic acid cycle
Up-Regulation
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