The overexpression of RXam1, a cassava gene coding for an RLK, confers disease resistance to Xanthomonas axonopodis pv. manihotis

Cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam) is a prevalent disease in all regions, where cassava is cultivated. CBB is a foliar and vascular disease usually controlled through host resistance. Previous studies have found QTLs explaining resistance to several X...

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Bibliographic Details
Published in:Planta 2018-04, Vol.247 (4), p.1031-1042
Main Authors: Tatis, Paula A. Díaz, Corzo, Mariana Herrera, Cabezas, Juan C. Ochoa, Cipagauta, Adriana Medina, Prías, Mónica A., Verdier, Valerie, Aguirre, Paul Chavarriaga, Carrascal, Camilo E. López
Format: Article
Language:English
Subjects:
Activin Receptors - genetics
Activin Receptors - metabolism
Agriculture
Bacteria
Bacterial artificial chromosomes
Biomedical and Life Sciences
Blight
Cassava
Disease control
Disease resistance
Disease Resistance - genetics
Ecology
Electrical resistivity
Forestry
Genes, Plant - genetics
Hybridization
Life Sciences
Manihot - genetics
ORIGINAL ARTICLE
Plant Diseases - microbiology
Plant Immunity - genetics
Plant Sciences
Polymerase Chain Reaction
Quantitative trait loci
Quantitative Trait Loci - genetics
Vascular diseases
Xanthomonas axonopodis
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Summary:Cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam) is a prevalent disease in all regions, where cassava is cultivated. CBB is a foliar and vascular disease usually controlled through host resistance. Previous studies have found QTLs explaining resistance to several Xam strains. Interestingly, one QTL called XM5 that explained 13% of resistance to XamCIO136 was associated with a similar fragment of the rice Xa21-resistance gene called PCR250. In this study, we aimed to further identify and characterize this fragment and its role in resistance to CBB. Screening and hybridization of a BAC library using the molecular marker PCR250 as a probe led to the identification of a receptor-like kinase similar to Xa21 and were called RXam1 (Resistance to Xam 1). Here, we report the functional characterization of susceptible cassava plants overexpressing RXam1. Our results indicated that the overexpression of RXam1 leads to a reduction in bacterial growth of XamCIO136. This suggests that RXAM1 might be implicated in strain-specific resistance to XamCIO136.
ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-018-2863-4