Seasonal changes in baseline corticosterone, association with innate immunity, and effects of confinement in free-ranging Eastern Box Turtles, Terrapene carolina carolina

• Published in: General and comparative endocrinology 2018-06, Vol.262, p.71-80
• Main Authors: West, Jessica M., Klukowski, Matthew
• Format: Article
• Language: English
• Subjects: Adrenal; Annual variation; Body condition; Stress; Triglyceride; Uric acid
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2018.03.016

•Baseline CORT concentrations were significantly higher in fall than in spring.•Baseline CORT levels in the summer of 2013 were higher than in the summer of 2014.•Baseline CORT levels were positively correlated with hemolysis titer.•CORT levels rose significantly in response to one hour of confinement.•Triglyceride levels were significantly higher in females than in males. Seasonal changes in glucocorticoids and their influence on the immune system are widespread in vertebrates, but whether this occurs in lesser studied taxa like turtles is unclear. The purpose of this study was to test for seasonal changes in baseline corticosterone (CORT), innate immunity, body condition, and metabolic factors (triglycerides, uric acid) in free-ranging Eastern Box Turtles (Terrapene carolina carolina), a species which is in decline across its range. In addition, the effect of handling and confinement on CORT levels was measured. In both years of the seasonal study, baseline CORT concentrations were significantly higher in fall than in spring, but summer CORT levels differed between the years. The annual variation in CORT levels may be related to weather conditions or associated with opportunistic mating. CORT levels of turtles confined for one hour were higher than turtles bled as quickly as possible, and unlike the seasonal study, females had higher baseline and post-stressor CORT levels than males. Baseline CORT levels were positively correlated with hemolysis titer, indicating a possible immunoenhancing effect of CORT. Triglycerides were positively correlated with body condition and were higher in females than males. The higher triglyceride level in females was likely associated with the energetic demands of reproduction and nesting. Males had a lower body condition in the fall than in both spring and summer, while females exhibited no seasonal differences in body condition. Uric acid and innate immunity measures failed to exhibit significant seasonal or sex related differences. Overall our findings of substantial annual, seasonal, and sexual variation in turtle physiology are consistent with the findings reported for other vertebrates and indicate the importance of incorporating these sources of variation into the design of future studies.