Demethylation of the CD133 Gene Is Frequently Detected in Advanced Colorectal Cancer

Background: Recently, it has been reported that colorectal carcinoma is created and propagated by a small number of undifferentiated tumorigenic CD133 + cells. Furthermore, it has been reported that CD133 expression is directly regulated by epigenetic modifications. Therefore, it is possible that CD...

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Published in:Anticancer research 2009-06, Vol.29 (6), p.2235-2237
Main Authors: HIBI, Kenji, SAKATA, Makiko, NEMOTO, Hiroshi, SANADA, Yutaka, KITAMURA, Yo-Hei, SAKURABA, Kazuma, SHIRAHATA, Atsushi, GOTO, Tetsuhiro, MIZUKAMI, Hiroki, SAITO, Mitsuo, ISHIBASHI, Kazuyoshi, KIGAWA, Gaku
Format: Article
Language:English
Subjects:
AC133 Antigen
Adenocarcinoma - genetics
Adenocarcinoma - secondary
Adult
Aged
Aged, 80 and over
Antigens, CD - genetics
Biological and medical sciences
Cell Differentiation
Colon - metabolism
Colon - pathology
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
DNA Methylation
Female
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression Regulation, Neoplastic
Glycoproteins - genetics
Humans
Lymphatic Metastasis
Male
Medical sciences
Middle Aged
Neoplasm Staging
Peptides - genetics
Polymerase Chain Reaction
Prognosis
Promoter Regions, Genetic
Rectum - metabolism
Rectum - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
Young Adult
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Summary:Background: Recently, it has been reported that colorectal carcinoma is created and propagated by a small number of undifferentiated tumorigenic CD133 + cells. Furthermore, it has been reported that CD133 expression is directly regulated by epigenetic modifications. Therefore, it is possible that CD133 expression by gene demethylation is related to colorectal carcinogenesis. Materials and Methods: The methylation status of the CD133 gene was examined in primary carcinomas and the corresponding normal tissues derived from 48 patients with colorectal cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated. Results: Demethylation of the CD133 gene was detected in 19 out of the 48 (40%) primary colon carcinomas, suggesting that the demethylation of CD133 is frequently observed in colorectal carcinomas. The clinicopathological data were correlated with the demethylation results. A significant difference was observed in the maximal tumor size (p=0.0222). Moreover, a trend was shown toward preferentially developing lymph node metastasis in demethylated tumors (p=0.214). Conclusion: CD133 was more frequently demethylated in advanced colorectal carcinomas.
ISSN:0250-7005
1791-7530