Effect of an exfoliating skincare regimen on the numbers of epithelial squames on the skin of operating theatre staff, studied by surface microscopy

• Published in: The Journal of hospital infection 2018-10, Vol.100 (2), p.190-194
• Main Authors: Wernham, A.G., Cain, O.L., Thomas, A.M.
• Format: Article
• Language: English
• Subjects: Adult; Animal Scales - anatomy & histology; Animals; Clean air; Desquamation; Emollients - administration & dosage; Female; Hand Disinfection - methods; Humans; Infection; Joint replacement; Male; Microscopy; Middle Aged; Operating Rooms; Skin - anatomy & histology; Skin - drug effects; Skin microscopy; Skin scales; Surgical Wound Infection - prevention & control; Volunteers
• ISSN: 0195-6701; 1532-2939
• DOI: 10.1016/j.jhin.2018.03.014

The shedding of epithelial squames (skin scales) by staff in operating theatre air is an important source of deep infection following joint replacement surgery. This is a serious complication, resulting in significant morbidity for the patient and substantial cost implications for healthcare systems. Much effort has been put into providing clean air in operating theatres, yet little attention has been given to reducing the shedding of surface skin scales at source. To develop a novel method for calculating surface skin scale density using surface microscopy, and to use it to evaluate the effect of a skincare regimen on operating theatre staff. Surface microscopy with Z-stacked imaging was used to visualize the effect of a skincare regimen involving three stages: washing with soap; exfoliation; and application of emollient. A USB microscope was then used in a field study to take images of the skin of operating theatre staff who applied the regimen to one lower limb the night before testing. The other limb was used as a control. Two blinded assessors analysed scale density. Z-stack images from the surface microscope enabled observations of the skincare regimen. The USB microscope also provided adequate images that enabled assessment of skin scale density. In the operating theatre staff, a 72.1% reduction in visible skin scales was observed following application of the skincare regimen. Further work is required to demonstrate how this effect correlates with dispersion of skin particles in a cleanroom, and subsequently in live operating theatre studies.