Effect of disinfectant residual on the interaction between bacterial growth and assimilable organic carbon in a drinking water distribution system

• Published in: Chemosphere (Oxford) 2018-07, Vol.202, p.586-597
• Main Authors: Li, Weiying, Zhang, Junpeng, Wang, Feng, Qian, Lin, Zhou, Yanyan, Qi, Wanqi, Chen, Jiping
• Format: Article
• Language: English
• Subjects: Assimilable organic carbon (AOC); Biological stability; Disinfectant effect; Drinking water distribution system; Intact cell concentrations (ICC)
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2018.03.056

Public health is threatened by deteriorated water quality due to bacterial regrowth and uncontrolled growth-related problems in drinking water distribution systems (DWDSs). To investigate the scope of this problem, a two-year field study was conducted in south China. The amount of assimilable organic carbon (AOC), total cell concentrations (TCC), and intact cell concentrations (ICC) of water samples were determined by flow cytometry. The results indicated that ICC was significantly correlated to AOC concentration when the chlorine concentration was less than 0.15 mg/L, and ICC was lower at chlorine concentrations greater than 0.15 mg/L, suggesting that free chlorine level had effect on AOC and ICC. To further analyze the effect of disinfectant on AOC and bacterial growth, we designed an orthogonal experiment with different dosages of two commonly used disinfectants, chlorine and chloramine. The results demonstrated that high concentrations of free chlorine (＞0.15 mg/L) and chloramine (＞0.4 mg/L) were associated with relatively low proportions of intact cells and cultivable bacteria. Compared with chlorine, chloramine tended to cause lower AOC level and intact cells, likely because the chlorinated disinfection byproducts (DBPs) were more easily absorbed by bacteria than the chloraminated DBPs. Based on the statistical analysis of 240 water samples, ICC was limited when AOC concentration was less than 135 μg/L, while temperature and the number of small-size particles showed positive effects on ICC (P＜0.05). We conclude that the use of chloramine and controlling particle numbers should be suitable strategies to limit bacterial regrowth. [Display omitted] •Only with chlorine less than 0.15 mg/L, ICC was significantly correlated to AOC.•Cl2 above 0.15 mg/L or NH2Cl above 0.4 mg/L was associated with low ICC proportion.•Chloramine tended to cause lower AOC level and intact cells proportions.•ICC was found to be limited when AOC was less than 135 μg/L.•Particles control and use of chloramine were proper ways to limit bacterial growth.