Qualitative and quantitative analysis of lipo‐alkaloids and fatty acids in Aconitum carmichaelii using LC–MS and GC–MS

Introduction Lipo‐alkaloid (LA) is a kind of C19‐norditerpenoid alkaloid in Aconitum species, which usually contains an aconitane skeleton and one or two fatty acid residues. Objective To qualify and quantify the fatty acids and lipo‐alkaloids in Aconitum carmichaelii. Methodology An ultra‐high perf...

Full description

Saved in:
Bibliographic Details
Published in:Phytochemical analysis 2018-07, Vol.29 (4), p.398-405
Main Authors: Liang, Ying, Yan, Guan‐Yu, Wu, Jian‐Lin, Zong, Xingxing, Liu, Zhongqiu, Zhou, Hua, Liu, Liang, Li, Na
Format: Article
Language:English
Subjects:
Aconitum carmichaelii
Alkaloids
Chains
Chromatography
Fatty acids
Gas chromatography
GC–MS
High performance liquid chromatography
LC–MS
lipo‐alkaloid
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Oleic acid
oxygenated fatty acid
Quadrupoles
Qualitative analysis
Qualitative research
Quantitative analysis
Residues
Scientific imaging
Spectroscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Introduction Lipo‐alkaloid (LA) is a kind of C19‐norditerpenoid alkaloid in Aconitum species, which usually contains an aconitane skeleton and one or two fatty acid residues. Objective To qualify and quantify the fatty acids and lipo‐alkaloids in Aconitum carmichaelii. Methodology An ultra‐high performance liquid chromatography‐triple quadrupole‐mass spectrometry (UHPLC‐QQQ‐MS) method was established to quantify LAs, while the free fatty acids were identified by gas chromatography–mass spectrometry (GC–MS) and ultra‐high performance liquid chromatography‐quadrupole‐time‐of‐flight‐mass spectrometry (UHPLC‐Q‐TOF‐MS). Results Six major LAs (1–6) containing linoleic, palmitic, and oleic acid residues as side chains were quantified. Eighteen fatty acids were determined by GC–MS, and 15 were detected as the side chains of LAs. The LAs containing these 15 fatty acid residues accounted for about a third of the total identified LAs. Moreover, the contents of linoleic, palmitic, and oleic acids were highest. In addition, 12 oxygenated fatty acids were also identified by UHPLC‐Q‐TOF‐MS for the first time. Conclusion The positive correlation between free fatty acids and LAs in A. carmichaelii indicated that the types and contents of LAs were influenced by free fatty acids. Table of Content 18 free fatty acids were determined by GC‐MS 12 oxygenated fatty acids were identified by UHPLC‐Q‐TOF‐MS for the first time A positive correlation between LAs and free fatty acids were revealed
ISSN:0958-0344
1099-1565
DOI:10.1002/pca.2760