Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions

Aim To investigate melatonin (MEL) levels in human dental pulp tissue (hDP) in type 2 diabetic (T2D) participants and the underlying molecular mechanisms of its effects in human dental pulp cells (hDPCs) under hyperglycaemia. Methodology The study included 16 healthy and 16 T2D participants who unde...

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Bibliographic Details
Published in:International endodontic journal 2018-10, Vol.51 (10), p.1149-1158
Main Authors: Milosavljević, A., DJukić, Lj, Toljić, B., Milašin, J., DŽeletović, B., Brković, B., Roganović, J.
Format: Article
Language:English
Subjects:
Aged
Antioxidants
Case-Control Studies
Cytotoxicity
Dental pulp
Dental Pulp - metabolism
Dentistry
Diabetes
Diabetes mellitus
Diabetes Mellitus, Type 2 - metabolism
Endodontics
Enzyme-Linked Immunosorbent Assay
Event-related potentials
Female
Histone acetyltransferase
histone acetyltransferase p300
Histone Acetyltransferases - metabolism
human dental pulp
Humans
Hyperglycemia
Hyperglycemia - metabolism
iNOS
Male
Melatonin
Melatonin - metabolism
Middle Aged
Molecular modelling
Nitric Oxide Synthase Type II - metabolism
Nitric-oxide synthase
p300 Protein
SOD activity
Statistics, Nonparametric
Superoxide dismutase
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Summary:Aim To investigate melatonin (MEL) levels in human dental pulp tissue (hDP) in type 2 diabetic (T2D) participants and the underlying molecular mechanisms of its effects in human dental pulp cells (hDPCs) under hyperglycaemia. Methodology The study included 16 healthy and 16 T2D participants who underwent vital pulp extirpation for hDP and four healthy participants undergoing third molar extraction for hDPCs analyses. MTT and NRU were used as tests for cytotoxicity. The pulp tissue levels of MEL, inducible NO synthase (iNOS) and superoxide dismutase (SOD) activity, as well as iNOS, histone acetyltransferase p300 (p300) and SOD activity levels in hDPCs incubated with MEL (0.1 and 1.0 mmol L−1) under normoglycaemia and hyperglycaemia were measured by enzyme‐linked immunosorbent assay. Comparisons between the two groups were made by unpaired t‐tests or Mann–Whitney test whilst the chi‐square test was used for dichotomous variables. To compare more groups, the Kruskal–Wallis test with Dunn's multiple comparison was used, whilst Spearman correlation was used to assess association between two variables. Results Melatonin was decreased (124.30 ± 21.6 vs. 240.0 ± 19.1 pg mL−1, P 
ISSN:0143-2885
1365-2591
DOI:10.1111/iej.12934