Bloom forming Alexandrium ostenfeldii (Dinophyceae) in shallow waters of the Åland Archipelago, Northern Baltic Sea

• Published in: Harmful algae 2009, Vol.8 (2), p.318-328
• Main Authors: Kremp, Anke, Lindholm, Tore, Dreßler, Nicole, Erler, Katrin, Gerdts, Gunnar, Eirtovaara, Sanna, Leskinen, Elina
• Format: Article
• Language: English
• Subjects: Alexandrium; Alexandrium ostenfeldii; Algae; Animal and plant ecology; Animal, plant and microbial ecology; Autoecology; Baltic Sea; Biological and medical sciences; Bioluminescent blooms; Brackish; Dinophyceae; Fundamental and applied biological sciences. Psychology; LSU and ITS rDNA; Marine; Morphology; Plant cytology, morphology, systematics, chorology and evolution; Plants and fungi; Salinity tolerance; Thallophyta; Toxicity
• ISSN: 1568-9883; 1878-1470
• DOI: 10.1016/j.hal.2008.07.004

In the past years, late summer blooms of the bioluminescent dinoflagellate Alexandrium ostenfeldii have become a recurrent phenomenon in coastal waters of the central and Northern Baltic Sea. This paper reports exceptionally high cell concentrations (10 5 to 10 6 cells L −1) of the species found during bioluminescent blooms in 2003 and 2004 in a shallow embayment of the Åland archipelago at the SW coast of Finland. Clonal cultures were established for morphological, molecular, toxicological and ecophysiological investigations to characterize the Finnish populations and compare them to other global A. ostenfeldii isolates. The Finnish isolates exhibited typical morphological features of A. ostenfeldii such as large size, a prominent ventral pore and an orthogonally bent first apical plate. However, unambiguous differentiation from closely related Alexandrium peruvianum was difficult due to considerable variation of sulcal anterior plate shapes. The Finnish strains were genetically distinct from other isolates of the species, but phylogenetic analyses revealed a close relationship to isolates from southern England and an A. peruvianum morphotype from the Spanish Mediterranean. Together these isolates formed a distinct clade which was separated from a clade containing other Northern European, North American and New Zealand populations. Toxin analyses confirmed the presence of the PSP toxins GTX2, GTX3 and STX in both Finnish isolates with GTX3 being the dominant toxin. Total relative PSP toxin contents were moderate, ranging from approximately 6 to 15 fmol cell −1 at local salinities of 5 and 10 psu, respectively. Spirolides were not detected. Salinity tolerance experiments showed that the Finnish isolates were well adapted to grow at the low salinities of the Baltic Sea. With a salinity range of approximately 6 to 20–25 psu, Baltic populations are physiologically distinct from their marine relatives. Vigorous production of different cyst types in the cultures suggest that cysts may play a crucial role in the survival and retainment of A. ostenfeldii populations in the Baltic Sea.