A Study on Relationship of Plasmid with Antibiotic Resistance in Thermophilic Campylobacter spp. Isolates from Environmental Samples

• Published in: Biotechnology (Faisalābād, Pakistan) Pakistan), 2008-12, Vol.7 (4), p.813-817
• Main Authors: Baserisale, M., Bahador, N.
• Format: Article
• Language: English
• Subjects: Campylobacter jejuni; Lari
• ISSN: 1682-296X
• DOI: 10.3923/biotech.2008.813.817

Susceptibility of thermophilic Campylobacter isolates from environmental samples to antibiotics was studied to investigate relation between occurrence of plasmid and antibiotic resistant character in Campylobacter jejuni. Antimicrobial susceptibility of environmental isolates of Camp, jejuni, Camp, coli and Camp, lari to antibiotics was assessed by disc diffusion method afterward, three strains of Camp, jejuni F44, P41 and W21 isolates were subjected to plasmid isolation and curing. The results on antibiotic susceptibility of Campylobacter isolates by disc diffusion method indicated that, all the isolates of Campylobacter were sensitive to ciprofloxacin and resistant to cefotaxime, cephalexin and ampicillin. In addition, more than 50% of the Camp, jejuni isolates were resistance to chloramphenicol and erythromycin. Plasmids were detected from 60, 50 and 80% of the Camp, jejuni, Camp, coli and Camp, lari isolates, respectively. The cured strain of Camp, jejuni F44 was sensitive to chloramphenicol and resistant to erythromycin. Hence, the most of Campylobacter isolates tested harbored plasmid and probably chloramphenicol resistant marker is plasmid mediated while, erythromycin resistant marker is chromosomally mediated. Therefore, probably gene resistant markers present in the plasmids can be transmitted among campylobacters in the environment and reach the human population by direct contact and via food products of animal origin.