Undersulfation of glycosaminoglycans induced by sodium chlorate treatment affects the progression of C6 rat glioma, in-vivo

• Published in: Brain research 2007-02, Vol.1131 (1), p.29-36
• Main Authors: Lobão-Soares, Bruno, Alvarez-Silva, Marcio, Mendes de Aguiar, Claudia B.N, Nicolau, Mauro, Trentin, Andrea G
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents - pharmacology; Biological and medical sciences; Brain Neoplasms - drug therapy; Brain Neoplasms - metabolism; Brain Neoplasms - physiopathology; Brain Tissue Transplantation - methods; Brain tumor; Cell adhesion; Cell Adhesion - drug effects; Cell Adhesion - physiology; Cell Line, Tumor; Cell proliferation; Cell Proliferation - drug effects; Chlorates - pharmacology; Disease Progression; Extracellular matrix; Extracellular Matrix - drug effects; Extracellular Matrix - metabolism; Glioma - drug therapy; Glioma - metabolism; Glioma - physiopathology; Glycosaminoglycans - metabolism; Herbicides - pharmacology; Male; Medical sciences; Neoplasm Invasiveness - physiopathology; Neoplasm Invasiveness - prevention & control; Neurology; Rats; Rats, Wistar; Sulfates - metabolism; Survival Rate; Treatment Outcome; Tumors of the nervous system. Phacomatoses
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2006.11.014

Abstract The stimulatory input of extracellular matrix (ECM) components has been implicated in the invasive properties of glioma cells. It has been demonstrated that undersulfation of glycosaminoglycans (GAGs) promoted by sodium chlorate (SC) treatment reduces C6 glioma cell proliferation and adhesion to ECM molecules, in-vitro. In the present study, the authors investigated the involvement of GAG undersulfation in glioma cell growth in the brain parenchyma. The in-vitro treatment of C6 cells with SC and subsequent intracerebral inoculation in vehicle containing SC resulted in a reduced proportion of animals bearing glioma and a reduced tumor mass diameter. It also promoted longer animal survival. Intracerebral inoculation of SC-treated C6 cells in vehicle without SC or the SC treatment after intracerebral implantation of untreated C6 cells did not result in any reduction of tumor growth. Alterations in clinical, hematological and behavioral parameters in the open field were observed near the point of death when tumors presented a greater size. The results suggest an important role of GAGs in glioma growth which possibly affects cell proliferation and/or interactions with the normal tissue environment.