Dna damage in human mononuclear cells induced by bacterial endotoxin

Damage to nuclear DNA in human peripheral blood mononuclear cells was studied after in vitro treatment with bacterial endotoxin by alkaline comet assay. It was found that LPS induced DNA damage as soon as over the first 30 min of incubation, while by the 4th hour of incubation DNA damage was found i...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2008-09, Vol.146 (3), p.301-303
Main Authors: Glukhov, I. L, Sirota, N. P, Kuznetsova, E. A
Format: Article
Language:English
Subjects:
Bacteria
Biomedical and Life Sciences
Biomedicine
Biophysics and Biochemistry
Cell Biology
Comet Assay
DNA comet assay
DNA Damage
Humans
Internal Medicine
Laboratory Medicine
Leukocytes, Mononuclear - drug effects
lipopolysaccharide
Lipopolysaccharides - pharmacology
mononuclear cells
NADPH Oxidases - antagonists & inhibitors
Onium Compounds - pharmacology
Pathology
Superoxide Dismutase - pharmacology
Superoxides - pharmacology
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Summary:Damage to nuclear DNA in human peripheral blood mononuclear cells was studied after in vitro treatment with bacterial endotoxin by alkaline comet assay. It was found that LPS induced DNA damage as soon as over the first 30 min of incubation, while by the 4th hour of incubation DNA damage was found in more than 95% cells. Exogenous superoxide dismutase completely protected DNA, which suggests that superoxide radical is the primary extracellular damaging agent. Polyphenol antioxidant (water-soluble lignin) and specific NADPH oxidase inhibitor (diphenyleneiodonium chloride) also produced a protective effect. Our results show that LPS-activated mononuclear cells can be used ex vivo as a convenient and adequate experimental system for evaluation of the efficiency of various substances in protection of lymphocyte DNA from the damaging effect of reactive oxygen species of LPS-stimulated monocytes.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-008-0275-3