Effect of high temperature stress on heat shock protein expression and antioxidant enzyme activity of two morphs of the mud crab Scylla paramamosain

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2018-09, Vol.223, p.10-17
• Main Authors: Liu, Zi-Ming, Zhu, Xian-Long, Lu, Jun, Cai, Wan-Jun, Ye, Ya-Ping, Lv, Yao-Ping
• Format: Article
• Language: English
• Subjects: Acclimatization; Animals; Antioxidant enzyme; Antioxidants - metabolism; Arthropod Proteins - metabolism; Brachyura - enzymology; Brachyura - physiology; Catalase - metabolism; Gills - enzymology; Gills - metabolism; Heat shock protein; Heat-Shock Proteins - metabolism; Hepatopancreas - enzymology; Hepatopancreas - metabolism; Hot Temperature; Morph; Scylla paramamosain; Stress, Physiological; Superoxide Dismutase - metabolism; Temperature
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpa.2018.04.016

The present study aimed to investigate the effect rapid temperature change from moderate temperature to high temperatures on heat shock protein (HSP) expression and antioxidant enzyme activities in mud crabs. Two mud crabs, one with one spine on the outer margin of the carpus of cheliped (Sp1) and another with two spines (Sp2), were acclimated at 25 °C and then transferred to a 33 °C environment, and HSP expression and antioxidant enzyme activity were assessed. HSP70 and HSP60 were markedly up-regulated in the gills and hepatopancreas of Sp1 and Sp2 after exposure to 35 °C. Exposure to 35 °C also significantly increased superoxide dismutase and catalase activity in the gills of Sp1 and Sp2, with transient changes in hepatopancreas. Apart from changes in antioxidant enzyme activities, HSPs were highly up-regulated after exposure to 37 °C, especially for HSP70. Gill HSP70 expression in Sp2 was 6.1 folds that of the control after 24 h of exposure to 37 °C, and 9.2 folds that of Sp1. Moreover, exposure to 37 °C further up-regulated HSP70 in the hepatopancreas of Sp1, compared to that in Sp2. Hence, HSPs play important roles in thermotolerance in S. paramamosain and Sp1 might have a stronger tolerance to hyperthermal stress than Sp2.