Ses proteins as possible targets for vaccine development against Staphylococcus epidermidis infections

The opportunistic pathogen Staphylococcus epidermidis is progressively involved in device-related infections. Since these infections involve biofilm formation, antibiotics are not effective. Conversely, a vaccine can be advantageous to prevent these infections. In view of vaccine development, predic...

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Bibliographic Details
Published in:The Journal of infection 2018-08, Vol.77 (2), p.119-130
Main Authors: Hofmans, Dorien, Khodaparast, Laleh, Khodaparast, Ladan, Vanstreels, Els, Shahrooei, Mohammad, Van Eldere, Johan, Van Mellaert, Lieve
Format: Article
Language:English
Subjects:
Biofilm
Biomaterial-related infection
Staphylococcus epidermidis
Surface protein
Vaccine
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Summary:The opportunistic pathogen Staphylococcus epidermidis is progressively involved in device-related infections. Since these infections involve biofilm formation, antibiotics are not effective. Conversely, a vaccine can be advantageous to prevent these infections. In view of vaccine development, predicted surface proteins were evaluated on their potential as a vaccine target. Immunoglobulins directed against S. epidermidis surface proteins SesB, M, O, Q and R were used to firstly affirm their surface location. Further, inhibitory effects of these IgGs on biofilm formation were determined in vitro on polystyrene and polyurethane surfaces and in vivo using a subcutaneous catheter mouse model. We also examined the opsonophagocytotic capacity of these IgGs. Surface localization of the five Ses proteins was demonstrated both for planktonic and sessile cells, though to a variable extent. Ses-specific IgGs added to planktonic cells had a variable inhibitory effect on cell adhesion to polystyrene, while only anti-SesO IgGs decreased cell attachment to polyurethane catheters. Although phagocytic killing was only obtained after opsonization with SesB-specific IgGs, a significant reduction of in vivo formed biofilms was observed after administration of SesB-, SesM- and SesO-specific IgGs. Regardless of their characterization or function, S. epidermidis surface proteins can be adequate targets for vaccine development aiming the prevention of device-related infections caused by invasive S. epidermidis strains.
ISSN:0163-4453
1532-2742
DOI:10.1016/j.jinf.2018.03.013