Gene delivery in the cornea: in vitro & ex vivo evaluation of solid lipid nanoparticle-based vectors

Inflammation is a process that underlies sight-threatening ocular surface diseases, and gene supplementation with the plasmid that encodes for p-IL10 will allow the sustained de novo synthesis of the cytokine to occur in corneal cells, and provide a long-term anti-inflammatory effect. This work desc...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2018-08, Vol.13 (15), p.1847-1854
Main Authors: Vicente-Pascual, Mónica, Albano, Andrea, Solinís, María Á, Serpe, Loredana, Rodríguez-Gascón, Alicia, Foglietta, Federica, Muntoni, Elisabetta, Torrecilla, Josune, Pozo-Rodríguez, Ana Del, Battaglia, Luigi
Format: Article
Language:English
Subjects:
Animals
Bioavailability
Cell Line
Cell Survival
Cornea - cytology
Cornea - metabolism
Drug delivery systems
Epithelium, Corneal - cytology
Epithelium, Corneal - metabolism
Eye Diseases - genetics
Eye Diseases - therapy
Gene therapy
Gene Transfer Techniques
Genetic Therapy
Herpes viruses
Humans
Infections
Inflammation
Interleukin-10 - genetics
Interleukin-10 - metabolism
Ligands
Lipids
Lipids - chemistry
Nanoparticles
Nanoparticles - chemistry
Particle Size
Plasmids
Polyvinyl alcohol
Rabbits
Transfection
Vectors (Biology)
Viral infections
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Summary:Inflammation is a process that underlies sight-threatening ocular surface diseases, and gene supplementation with the plasmid that encodes for p-IL10 will allow the sustained de novo synthesis of the cytokine to occur in corneal cells, and provide a long-term anti-inflammatory effect. This work describes the development of solid lipid nanoparticle systems for the delivery of p-IL10 to transfect the cornea. In vitro, vectors showed suitable features as nonviral vectors (size, ζ-potential, DNA binding, protection and release), and they were able to enter and transfect human corneal epithelial cells. Ex vivo, the vectors were found to transfect the epithelium, the stroma and the endothelium in rabbit corneal explants. Distribution of gene expression within the cell layers of the cornea depended on the composition of the four vectors evaluated. Solid lipid nanoparticle-based vectors are promising gene delivery systems for corneal diseases, including inflammation.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2018-0112