Metallothionein in Hermetia illucens (Linnaeus, 1758) larvae (Diptera: Stratiomyidae), a potential biomarker for organic waste system

Black soldier fly, Hermetia illucens (Linnaeus, 1758), is an important economic fly as its larvae can be used for recycling organic waste, such as food waste and manure. H. illucens larvae (BSFL) could uptake Cd from substrates and accumulate it inside bodies, which need to be monitored during waste...

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Bibliographic Details
Published in:Environmental science and pollution research international 2018-02, Vol.25 (6), p.5379-5385
Main Authors: Wang, Xiaoyun, Gao, Qiao, Liu, Xinhui, Wang, Xiao-Ping, Lei, Chaoliang, Sayed, Waheed A. A., Zhu, Fen
Format: Article
Language:English
Subjects:
Amino acids
Aquatic Pollution
Atmospheric Protection/Air Quality Control/Air Pollution
Biomarkers
Cadmium
cysteine
Detoxification
dose response
Earth and Environmental Science
Ecotoxicology
Environment
Environmental Chemistry
Environmental Health
Environmental monitoring
Environmental science
Food contamination
Food waste
genes
Genetic transformation
Heavy metals
Hermetia illucens
Homeostasis
insects
Larvae
Meals
Metallothionein
Next-generation sequencing
Organic wastes
Research Article
sequence analysis
Substrates
Waste treatment
Waste Water Technology
Water Management
Water Pollution Control
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Summary:Black soldier fly, Hermetia illucens (Linnaeus, 1758), is an important economic fly as its larvae can be used for recycling organic waste, such as food waste and manure. H. illucens larvae (BSFL) could uptake Cd from substrates and accumulate it inside bodies, which need to be monitored during waste treatment. Metallothionein (MT) usually serve as biomarker because of its role in metal homeostasis, detoxification, and dose response of heavy metals. Therefore, a MT gene was cloned from H. illucens ( HIMT ) that encoded 40 amino acids with typical cysteine rich features, which had a high sequence identity with other insect MTs. The expression of HIMT and total MT protein was measured in BSFL fed by meals spiked with gradient dose of Cd (0, 5, 50, 500 mg/kg) for 24, 48, 72, and 96 h, respectively. Dose-associated response of HIMT and total MT were found and the possible correlative range of Cd was from 5 to 50 mg/kg. The expression of HIMT might be a potential biomarker for monitoring Cd contamination by H. illucens in terrestrial organic matters, which might further apply in waste transformation system.
ISSN:0944-1344
1614-7499
DOI:10.1007/s11356-017-0856-7