A Multifunctional Chemical Probe for the Measurement of Local Micropolarity and Microviscosity in Mitochondria

The measurement of physicochemical parameters in living cells can provide information on individual cellular organelles, helping us to understand subcellular function in health and disease. While organelle‐specific chemical probes have allowed qualitative evaluation of microenvironmental variations,...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2018-07, Vol.57 (29), p.8891-8895
Main Authors: Jiménez‐Sánchez, Arturo, Lei, Eric K., Kelley, Shana O.
Format: Article
Language:English
Subjects:
cellular microviscosity
cellular polarity
Coumarins - chemistry
Fluorescence
Fluorescent Dyes - chemistry
fluorescent probes
HeLa Cells
Humans
Microscopy, Fluorescence
Mitochondria
Mitochondria - chemistry
Mitochondria - ultrastructure
Morphology
Optical Imaging
Organelles
Organic chemistry
Peptides - chemistry
Quinoxalines - chemistry
Spectrometry, Fluorescence
Viscosity
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Summary:The measurement of physicochemical parameters in living cells can provide information on individual cellular organelles, helping us to understand subcellular function in health and disease. While organelle‐specific chemical probes have allowed qualitative evaluation of microenvironmental variations, the simultaneous quantification of mitochondrial local microviscosity (ηm) and micropolarity (ϵm), along with concurrent structural variations, has remained an unmet need. Herein, we describe a new multifunctional mitochondrial probe (MMP) for simultaneous monitoring of ηm and ϵm by fluorescence lifetime and emission intensity recordings, respectively. The MMP enables highly precise measurements of ηm and ϵm in the presence of a variety of agents perturbing cellular function, and the observed changes can also be correlated with alterations in mitochondrial network morphology and motility. This strategy represents a promising tool for the analysis of subtle changes in organellar structure. A fluorescent probe enables highly precise, quantitative measurements of local mitochondrial microviscosity and micropolarity in the presence of a variety of agents perturbing cellular function. The observed changes could also be correlated with alterations in mitochondrial network morphology and motility.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201802796