Cytoprotective Agent in Lactobacillus bulgaricus Extracts

Adenosine 5'-diphosphoribose (ADP-ribose) has been identified as a significant contributor to the anti-cytotoxic activity of Lactobacillus bulgaricus extracts. Although the biological activities associated with the administration of probiotic bacteria and components thereof are sometimes attrib...

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Bibliographic Details
Published in:Current microbiology 2007-02, Vol.54 (2), p.131-135
Main Authors: Johns, Paul, Pereira, Suzette L, Leonard, Amanda E, Mukerji, Pradip, Shalwitz, Robert A, Dowlati, Lobat, Phillips, Rosalyn R, Bergana, Marti S, Holton, Jason D, Das, Tapas
Format: Article
Language:English
Subjects:
Adenosine diphosphate
Adenosine Diphosphate Ribose - chemistry
Adenosine Diphosphate Ribose - isolation & purification
Adenosine Diphosphate Ribose - metabolism
Adenosine Diphosphate Ribose - pharmacology
Bacteria
Cell Line
Chromatography, High Pressure Liquid
Cycloheximide - toxicity
Cytotoxicity
Epithelial Cells - drug effects
Lactobacillus - metabolism
Lactobacillus bulgaricus
Liquid chromatography
Lung - cytology
Magnetic Resonance Spectroscopy
NMR
Nuclear magnetic resonance
Probiotics - metabolism
Tumor Necrosis Factor-alpha - toxicity
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Summary:Adenosine 5'-diphosphoribose (ADP-ribose) has been identified as a significant contributor to the anti-cytotoxic activity of Lactobacillus bulgaricus extracts. Although the biological activities associated with the administration of probiotic bacteria and components thereof are sometimes attributed to the peptidoglycans that comprise a substantial portion of the Gram-positive bacterial cell wall, we found that the beta-nicotine adenine dinucleotide (NAD) hydrolysis product ADP-ribose was a significant contributor to the observed anti-cytotoxicity in our L. bulgaricus extracts. The ADP-ribose was isolated, identified, and quantitated by high performance liquid chromatography (HPLC) and by nuclear magnetic resonance (NMR) spectroscopy. ADP-ribose levels as low as 5 mg/L exhibited a measurable inhibition of tumor necrosis factor alpha (TNF-α) mediated cytotoxicity in an in vitro cell assay, whereas the ADP-ribose content of the L. bulgaricus extracts often exceeded 5 mg/g dry weight.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-006-0256-6