Evaluating the combinative effects on human lymphocyte DNA damage induced by Ultraviolet ray C plus 1.8GHz microwaves using comet assay in vitro

• Published in: Toxicology (Amsterdam) 2007-04, Vol.232 (3), p.311-316
• Main Authors: Baohong, Wang, Lifen, Jin, Lanjuan, Li, Jianlin, Lou, Deqiang, Lu, Wei, Zheng, Jiliang, He
• Format: Article
• Language: English
• ISSN: 0300-483X
• DOI: 10.1016/j.tox.2007.01.019

The objective of this study was to observe whether 1.8GHz microwaves (MW) (SAR, 3W/kg) exposure can influence human lymphocyte DNA damage induced by ultraviolet ray C (UVC). The lymphocytes, which were from three young healthy donors, were exposed to 254nm UVC at the doses of 0.25, 0.5, 0.75, 1.0, 1.5and 2.0Jm super(-) super(2), respectively. The lymphocytes were irradiated by 1.8GHz MW (SAR, 3W /kg) for 0, 1.5 and 4h. The combinative exposure of UVC plus MW was conducted. The treated cells were incubated for 0, 1.5 and 4h. Finally, comet assay was used to measure DNA damage of above treated lymphocytes. The results indicated that the difference of DNA damage induced between MW group and control group was not significant (P>0.05). The MTLs induced by UVC were 1.71+/-0.09, 2.02+/-0.08, 2.27+/-0.17, 2.27+/-0.06, 2.25+/-0.12, 2.24+/-0.11 mu m, respectively, which were significantly higher than that (0.96+ /-0.05 mu m) of control (P