Prevalence of factor V leiden, MTHFR C677T and MTHFR A1298C polymorphisms in patients with deep vein thrombosis in Central Iran

• Published in: Molecular biology reports 2018-08, Vol.45 (4), p.621-624
• Main Authors: Ehsani, Majid, Imani, Aida, Moravveji, Alireza
• Format: Article
• Language: English
• Subjects: Adult; Aged; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Case-Control Studies; Coagulation factors; Cross-Sectional Studies; Factor V; Factor V - genetics; Factor V - metabolism; Female; Gene Frequency; Gene polymorphism; Genetic markers; Genetic Predisposition to Disease - genetics; Genotype; Geriatrics; Histology; Homocystinuria - genetics; Humans; Immobilization; Iran - epidemiology; Life Sciences; Male; Methylenetetrahydrofolate reductase; Methylenetetrahydrofolate Reductase (NADPH2) - deficiency; Methylenetetrahydrofolate Reductase (NADPH2) - genetics; Methylenetetrahydrofolate Reductase (NADPH2) - metabolism; Middle Aged; Morphology; Muscle Spasticity - genetics; Mutation; Original Article; Patients; Polymerase chain reaction; Polymorphism; Polymorphism, Single Nucleotide - genetics; Prevalence; Psychotic Disorders - genetics; Restriction fragment length polymorphism; Risk Factors; Thrombosis; Venous Thrombosis - etiology; Venous Thrombosis - genetics
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-018-4201-0

Deep vein thrombosis (DVT) is a common disease, especially among elderly patients, which is associated with high costs of treatment and high rates of recurrence. The risk factors for venous thrombosis are primarily related to hypercoagulability, which can be genetic or acquired, or because of immobilization and venous stasis. Among relevant genetic markers are a number of common polymorphisms and mutations in the genes coding for Factor V leiden and methylenetetrahydrofolate reductase. Differential associations of these polymorphisms have been reported in different populations with DVT due to ethnic variations. However, no study has been reported with respect to these polymorphisms in DVT in Iran. Thus, the aim of the present study is to determine the prevalence of FVL, MTHFR C677T and MTHFR A1298C gene polymorphisms in patients with DVT in central Iran. In the present cross-sectional study, a total of 100 patients with first and recurrent episodes of DVT and age less than 70 years were recruited during 2016–2017. Blood sample was collected from the recruited patients and FVL mutation was screened using ARMS-PCR method, MTHFR C677T and MTHFR A1298C mutations were screened using PCR-RFLP method. The results revealed that MTHFR A1298C gene polymorphism in both homozygote and heterozygote form was found to be most frequent i.e. 77% among cases, followed by MTHFR C677T (67%) and FVL (17%). The study highlights the importance of screening of these genetic markers among patients with DVT in this region.