Pattern formation of stains from dried drops to identify spermatozoa motility

[Display omitted] •Stains from drying suspension drops may be used to detect cell motility.•Lacunarity analysis classifies deposits containing motile and non-motile sperm cells.•A method to detect motility in a fluid might be used in other biological systems. We study how cell motility affects the s...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2018-09, Vol.169, p.486-493
Main Authors: Ríos-Ramírez, Maricarmen, Reyes-Figueroa, A.D., Ruiz-Suárez, J.C., González-Gutiérrez, Jorge
Format: Article
Language:English
Subjects:
Droplets
Motility
Pattern formation
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Summary:[Display omitted] •Stains from drying suspension drops may be used to detect cell motility.•Lacunarity analysis classifies deposits containing motile and non-motile sperm cells.•A method to detect motility in a fluid might be used in other biological systems. We study how cell motility affects the stains left by the evaporation of droplets of a biofluid suspension containing mouse spermatozoa. The suspension, which contains also a high concentration of salts usually needed by motile cells, forms, upon drying, a crystallized pattern. We examine the structural characteristics of such patterns by optical microscopy. The analysis reveals that cell motility affects the formation of elongated crystals with lateral tips, as well as the creation of interlocked aggregates. We prove that a lacunarity algorithm based on polar symmetry, distinguishes among deposits generated by motile and non-motile cells with an accuracy greater than 95%.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2018.05.033