Enzyme–Polymer Conjugates to Enhance Enzyme Shelf Life in a Liquid Detergent Formulation

Herein, the synthesis of enzyme–polymer conjugates is reported. Four different activated polymers (mPEG‐aldehyde, mPEG‐NHS, maltodextrin‐aldehyde, carboxymethyl cellulose aldehyde) are conjugated to the surface of protease, α‐amylase, and lipase using two different strategies (reductive amination an...

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Bibliographic Details
Published in:Macromolecular bioscience 2018-07, Vol.18 (7), p.e1800095-n/a
Main Authors: Kübelbeck, Sonja, Mikhael, Jules, Keller, Harald, Konradi, Rupert, Andrieu‐Brunsen, Annette, Baier, Grit
Format: Article
Language:English
Subjects:
Alkylation
Amination
Carboxymethyl cellulose
Carboxymethylcellulose
Cellulose
Chemical modification
Chemical synthesis
Conjugates
Detergents
differential scanning calorimetry (DSC)
Enzymatic activity
Enzyme activity
Enzymes
Fractionation
Lipase
Maltodextrin
modification
MPEG encoders
Organic chemistry
Polymers
Shelf life
stabilization
surfactants
Thermal stability
Video compression
α-Amylase
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Summary:Herein, the synthesis of enzyme–polymer conjugates is reported. Four different activated polymers (mPEG‐aldehyde, mPEG‐NHS, maltodextrin‐aldehyde, carboxymethyl cellulose aldehyde) are conjugated to the surface of protease, α‐amylase, and lipase using two different strategies (reductive amination and alkylation with NHS‐activated acid). Although the chemical modification of the enzymes is accompanied by losses in enzyme activity (maximum loss 40%), the covalent attachment of polymers increases the thermal stability and the stability in a standard detergent formulation compared to the unmodified enzymes. The enzyme–polymer conjugates are characterized by asymmetrical‐flow field‐flow fractionation and differential scanning microcalorimetry. Furthermore, it is demonstrated that conjugated enzymes still show performance in a real washing process. Enzyme–polymer conjugates show a potential as a stabilizing system for enzymes in detergents. The synthesis of enzyme–polymer conjugates is reported. Four different activated polymers are conjugated to the surface of three enzymes using two different strategies. Although the chemical modification of the enzymes is accompanied by slight losses in enzyme activity, the covalent attachment of polymers increases the thermal stability and the stability in a standard detergent formulation compared to the unmodified enzymes.
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.201800095