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Transcriptomic study on persistence and survival of Listeria monocytogenes following lethal treatment with nisin

•Persister Listeria monocytogenes cells form following exposure to lethal nisin concentrations.•RNA-Seq revealed multiple systems associated with the persister population.•Exposure of L. monocytogenes to lethal nisin concentrations alters transcriptional regulation.•RNA-Seq data were validated by re...

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Bibliographic Details
Published in:Journal of global antimicrobial resistance. 2018-12, Vol.15, p.25-31
Main Authors: Wu, Shuyan, Yu, Pak-Lam, Wheeler, Dave, Flint, Steve
Format: Article
Language:English
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Summary:•Persister Listeria monocytogenes cells form following exposure to lethal nisin concentrations.•RNA-Seq revealed multiple systems associated with the persister population.•Exposure of L. monocytogenes to lethal nisin concentrations alters transcriptional regulation.•RNA-Seq data were validated by reverse transcription quantitative PCR. The aim of this study was to determine gene expression associated with the persistence of a Listeria monocytogenes stationary-phase population when facing lethal nisin treatment. RNA-Seq analysis was used for gene expression profiling of persister cells in nutrient-rich medium (persister TN) compared with untreated cells (non-persister). The results were confirmed using reverse transcription quantitative PCR (RT-qPCR). Functional genes associated with the persister population were identified in multiple systems, such as heat-shock-related stress response, cell wall synthesis, ATP-binding cassette (ABC) transport system, phosphotransferase system (PTS) and SOS/DNA repair. This study pointed to genetic regulation of persister cells exposed to lethal nisin concentrations and provides some insight into possible mechanisms of impeding bacterial persistence.
ISSN:2213-7165
2213-7173
DOI:10.1016/j.jgar.2018.06.003