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Transcriptomic study on persistence and survival of Listeria monocytogenes following lethal treatment with nisin
•Persister Listeria monocytogenes cells form following exposure to lethal nisin concentrations.•RNA-Seq revealed multiple systems associated with the persister population.•Exposure of L. monocytogenes to lethal nisin concentrations alters transcriptional regulation.•RNA-Seq data were validated by re...
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Published in: | Journal of global antimicrobial resistance. 2018-12, Vol.15, p.25-31 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | •Persister Listeria monocytogenes cells form following exposure to lethal nisin concentrations.•RNA-Seq revealed multiple systems associated with the persister population.•Exposure of L. monocytogenes to lethal nisin concentrations alters transcriptional regulation.•RNA-Seq data were validated by reverse transcription quantitative PCR.
The aim of this study was to determine gene expression associated with the persistence of a Listeria monocytogenes stationary-phase population when facing lethal nisin treatment.
RNA-Seq analysis was used for gene expression profiling of persister cells in nutrient-rich medium (persister TN) compared with untreated cells (non-persister). The results were confirmed using reverse transcription quantitative PCR (RT-qPCR).
Functional genes associated with the persister population were identified in multiple systems, such as heat-shock-related stress response, cell wall synthesis, ATP-binding cassette (ABC) transport system, phosphotransferase system (PTS) and SOS/DNA repair.
This study pointed to genetic regulation of persister cells exposed to lethal nisin concentrations and provides some insight into possible mechanisms of impeding bacterial persistence. |
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ISSN: | 2213-7165 2213-7173 |
DOI: | 10.1016/j.jgar.2018.06.003 |