Comparison of peptide and superantigen-induced anergy in a peptide-specific polyclonal human T cell line

T cells recognizing tetanus toxin peptide ‘p2’ (sequence 830–844) raised in HLA DR6 individuals preferentially express Vβ2 in the TCR. A p2-specific T cell line (60% Vβ2+) was used to compare peptide and superantigen [toxic shock syndrome toxin-1 (TSST-1)]-induced clonal anergy. Many experiments con...

Full description

Saved in:
Bibliographic Details
Published in:International immunology 1995-07, Vol.7 (7), p.1057-1063
Main Authors: Chu, N. Randall, Quaratino, Sonia, Feldmann, Marc, Londei, Marco
Format: Article
Language:English
Subjects:
Amino Acid Sequence
autoimmunity
Bacterial Toxins
Base Sequence
Cell Line
Clonal Anergy
Clostridium tetani
Dose-Response Relationship, Immunologic
Enterotoxins - immunology
Epitopes
Humans
Immune Tolerance
Lymphocyte Activation
Molecular Sequence Data
Peptide Fragments - immunology
Peptide Fragments - pharmacology
Receptor-CD3 Complex, Antigen, T-Cell - immunology
Staphylococcus aureus - immunology
superantigen
Superantigens - pharmacology
T-Lymphocytes - immunology
Tetanus Toxin - immunology
tolerance
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:T cells recognizing tetanus toxin peptide ‘p2’ (sequence 830–844) raised in HLA DR6 individuals preferentially express Vβ2 in the TCR. A p2-specific T cell line (60% Vβ2+) was used to compare peptide and superantigen [toxic shock syndrome toxin-1 (TSST-1)]-induced clonal anergy. Many experiments consistently revealed that the degree of ‘tolerance’ or ‘clonal anergy’ induced by peptide was greater than with the superantigen TSST-1. These results are of interest in a number of contexts. First they suggest that using superantigens or anti-Vβ to delete the majority population of T cells may not be sufficient to diminish an autoimmune response. Secondly, the results indicate that induction of anergy of a large proportion of peptide-specific T cells does not lead to a suppressive bystander effect on the remaining responsive T cells. These results emphasize the need to define the dominant autoantigenic epitopes in human autoimmune diseases, since peptide based therapy such as the use of peptide analogues to induce anergy or a change in cytokine profile, is possibly more effective in controlling undesired immune responses than the use of non-antigen, TCR-directed approaches such as superantigens.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/7.7.1057